Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-azobenzene sulfonic acid | 63% residual activity at 5 mM | Deinococcus radiophilus | |
Ag+ | enzyme activity is moderately reduced (50%) by 1 mM | Deinococcus radiophilus | |
Cd2+ | enzyme activity is drastically reduced (70%) by 1 mM | Deinococcus radiophilus | |
Cu2+ | enzyme activity is drastically reduced (70%) by 1 mM | Deinococcus radiophilus | |
Glyoxal | 93% residual activity at 5 mM | Deinococcus radiophilus | |
Hg2+ | enzyme activity is drastically reduced (70%) by 1 mM | Deinococcus radiophilus | |
Iodine | 86% residual activity at 5 mM | Deinococcus radiophilus | |
additional information | not inhibited by 1-chloro-2,4-dinitrobenzene | Deinococcus radiophilus | |
N-ethylmaleimide | 46% residual activity at 5 mM | Deinococcus radiophilus | |
Zn2+ | enzyme activity is drastically reduced (70%) by 1 mM | Deinococcus radiophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
NADPH | in 50 mM potassium phosphate buffer (pH 7.0), at 22°C | Deinococcus radiophilus | |
0.0302 | - |
NADH | in 50 mM potassium phosphate buffer (pH 7.0), at 22°C | Deinococcus radiophilus | |
0.463 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | in 50 mM potassium phosphate buffer (pH 7.0), at 22°C | Deinococcus radiophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63100 | - |
PAGE | Deinococcus radiophilus |
72200 | - |
gel filtration | Deinococcus radiophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin disulfide + NADH + H+ | Deinococcus radiophilus | - |
thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide + NADH + H+ | Deinococcus radiophilus ATCC 27603 | - |
thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Deinococcus radiophilus | - |
thioredoxin + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Deinococcus radiophilus ATCC 27603 | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiophilus | - |
- |
- |
Deinococcus radiophilus ATCC 27603 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, 2'5'-ADP Sepharose 4B affinity column chromatography, and Sephadex G-100 gel filtration | Deinococcus radiophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.77 | - |
enzyme from cell-free extract, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C | Deinococcus radiophilus |
367.7 | - |
after 478fold purification, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C | Deinococcus radiophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Deinococcus radiophilus | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Deinococcus radiophilus ATCC 27603 | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Deinococcus radiophilus | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Deinococcus radiophilus ATCC 27603 | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
thioredoxin disulfide + NADH + H+ | - |
Deinococcus radiophilus | thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide + NADH + H+ | - |
Deinococcus radiophilus ATCC 27603 | thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Deinococcus radiophilus | thioredoxin + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Deinococcus radiophilus ATCC 27603 | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 000, SDS-PAGE | Deinococcus radiophilus |
Synonyms | Comment | Organism |
---|---|---|
EC 1.6.4.5 | formerly | Deinococcus radiophilus |
L-TrxR | - |
Deinococcus radiophilus |
TrxR | - |
Deinococcus radiophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the enzyme is stable at temperatures ranging from 4°C to 70°C for 30 min. However, the enzyme activity is drastically reduced at 80°C and completely inactivated at 90°C | Deinococcus radiophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the purified enzyme is stable at pH 6.0-7.0 and relatively stable even at pH 8.0-9.0, but quite unstable below pH 5.0 | Deinococcus radiophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Deinococcus radiophilus | |
NADH | - |
Deinococcus radiophilus | |
NADPH | - |
Deinococcus radiophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus radiophilus | isoelectric focusing | - |
4.5 |