Literature summary for 1.8.1.9 extracted from

Rengby, O.; Cheng, Q.; Vahter, M.; Joernvall, H.; Arner, E.S.
Highly active dimeric and low-activity tetrameric forms of selenium-containing rat thioredoxin reductase 1 (2009), Free Radic. Biol. Med., 46, 893-904.

Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0032	-	thioredoxin disulfide	purified, recombinant, tetrameric enzyme	Rattus norvegicus
0.0036	-	thioredoxin disulfide	purified, recombinant, dimeric enzyme	Rattus norvegicus
0.139	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, tetrameric enzyme	Rattus norvegicus
0.2156	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, dimeric enzyme	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
selenium	TrxR1 is a selenoprotein that contains a selenocysteine residue at the penultimate C-terminal	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54386	-	2 * 54386, highly active enzyme form, calculated from amino acid sequence	Rattus norvegicus
150000	-	highly active enzyme form, gel filtration	Rattus norvegicus
250000	-	low-activity enzyme form, gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	O89049	-	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP Sepharose column chromatography and phenylarsine oxide Sepharose column chromatography	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+	-	Rattus norvegicus	2-nitro-5-thiobenzoate + NADP+	-	?
thioredoxin disulfide + NADPH + H+	-	Rattus norvegicus	thioredoxin + NADP+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 54386, highly active enzyme form, calculated from amino acid sequence	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
thioredoxin reductase 1	-	Rattus norvegicus
TrxR1	-	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
33.08	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, tetrameric enzyme	Rattus norvegicus
47	73	thioredoxin disulfide	purified, recombinant, tetrameric enzyme	Rattus norvegicus
106.3	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, dimeric enzyme	Rattus norvegicus
114.1	-	thioredoxin disulfide	purified, recombinant, dimeric enzyme	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Rattus norvegicus
NADPH	-	Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
183.3	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, tetrameric enzyme	Rattus norvegicus
495	-	5,5'-dithiobis(2-nitrobenzoic acid)	purified, recombinant, dimeric enzyme	Rattus norvegicus
14920	-	thioredoxin disulfide	purified, recombinant, tetrameric enzyme	Rattus norvegicus
31700	-	thioredoxin disulfide	purified, recombinant, dimeric enzyme	Rattus norvegicus

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Release 2023.1 (January 2023)