Literature summary for 1.8.1.9 extracted from

Wang, Y.; Zhang, X.; Liu, Q.; Ai, C.; Mo, H.; Zeng, J.
Expression, purification and molecular structure modeling of thioredoxin (Trx) and thioredoxin reductase (TrxR) from Acidithiobacillus ferrooxidans (2009), Curr. Microbiol., 59, 35-41.

Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Acidithiobacillus ferrooxidans

Protein Variants

Protein Variants	Comment	Organism
C142A	mutant shows reduced reductase activity compared to the wild type enzyme	Acidithiobacillus ferrooxidans
C142S	mutant shows reduced reductase activity compared to the wild type enzyme	Acidithiobacillus ferrooxidans
C145A	mutant shows reduced reductase activity compared to the wild type enzyme	Acidithiobacillus ferrooxidans
C145S	mutant shows reduced reductase activity compared to the wild type enzyme	Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	SDS-PAGE	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	strain ATCC 23270	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity column chromatography	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.1	-	mutant enzyme C145S, in 50 mM potassium phosphate (pH 7.0), at 20°C	Acidithiobacillus ferrooxidans
5.5	-	mutant enzyme C142S, in 50 mM potassium phosphate (pH 7.0), at 20°C	Acidithiobacillus ferrooxidans
5.6	-	mutant enzyme C145A, in 50 mM potassium phosphate (pH 7.0), at 20°C	Acidithiobacillus ferrooxidans
6.4	-	mutant enzyme C142A, in 50 mM potassium phosphate (pH 7.0), at 20°C	Acidithiobacillus ferrooxidans
29.7	-	wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 20°C	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thioredoxin disulfide + NADPH + H+	-	Acidithiobacillus ferrooxidans	thioredoxin + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
thioredoxin reductase	-	Acidithiobacillus ferrooxidans
TrxR	-	Acidithiobacillus ferrooxidans

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Acidithiobacillus ferrooxidans
NADPH	-	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)