Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl-1-propyl-2-imidazolyl disulfide | - |
Bos taurus | |
1-methyl-1-propyl-2-imidazolyl disulfide | - |
Homo sapiens | |
curcumin | - |
Bos taurus | |
curcumin | - |
Homo sapiens | |
Hg2+ | - |
Bos taurus | |
Hg2+ | - |
Homo sapiens | |
additional information | black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively. Kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) | Homo sapiens | |
theaflavin | - |
Bos taurus | |
theaflavin-3'-monogallate | - |
Bos taurus | |
theaflavin-3,3'-digallate | - |
Bos taurus | |
theaflavin-3-monogallate | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Homo sapiens |
110000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | O62768 | - |
- |
Homo sapiens | Q16881 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
EAhy 926 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Bos taurus | 2-nitro-5-thiobenzoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Homo sapiens |
thioredoxin reductase | - |
Bos taurus |
TrxR1 | - |
Homo sapiens |
TrxR1 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively | Homo sapiens | additional information | |
0.018 | - |
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 | Bos taurus | theaflavin-3-monogallate | |
0.0191 | - |
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 | Bos taurus | theaflavin-3'-monogallate | |
0.0216 | - |
in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 | Bos taurus | theaflavin-3,3'-digallate | |
0.06 | - |
IC50 above 0.06 mM, in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 | Bos taurus | theaflavin |