Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzymes with or without NADP+, using 12-15% (w/v) polyethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4, and 50 mM sodium acetate (pH 4.6) | Saccharolobus solfataricus |
wild type enzyme in complex with NADP+ and mutant enzyme C147A, using 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4 and 50 mM sodium acetate (pH 4.6) | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C147A | the mutant exhibits a marginal NADH oxidase activity with FAD canonically bound to the enzyme | Saccharolobus solfataricus |
C147A | in the active site of the C147A mutant, which exhibits a marginal NADH oxidase activity, the FAD is canonically bound to the enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W27 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
disulfide oxidase + NADPH + H+ | - |
Saccharolobus solfataricus | ? | - |
? | |
additional information | TrxRB3 also displays NADH oxidase activity | Saccharolobus solfataricus | ? | - |
? | |
additional information | TrxRB3 is endowed with an additional NADH oxidase activity | Saccharolobus solfataricus | ? | - |
? | |
thioredoxin + NADP+ | - |
Saccharolobus solfataricus | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Saccharolobus solfataricus | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Saccharolobus solfataricus |
TrxRB3 | - |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Saccharolobus solfataricus | |
NADPH | - |
Saccharolobus solfataricus |