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Literature summary for 1.8.1.9 extracted from

  • Angelucci, F.; Miele, A.E.; Boumis, G.; Dimastrogiovanni, D.; Brunori, M.; Bellelli, A.
    Glutathione reductase and thioredoxin reductase at the crossroad: the structure of Schistosoma mansoni thioredoxin glutathione reductase (2008), Proteins, 72, 936-945.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00637
-
thioredoxin disulfide full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
0.114
-
5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
0.71
-
5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
SDS-PAGE Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Schistosoma mansoni ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 90000, SDS-PAGE Schistosoma mansoni

Synonyms

Synonyms Comment Organism
TGR
-
Schistosoma mansoni
thioredoxin glutathione reductase chimeric enzyme able to transport reducing equivalents from NADPH to both GSH/GSSG and thioredoxin systems via the fusion of the thioredxin reductase and glutathione reductase domains Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
5,5'-dithiobis(2-nitrobenzoic acid) truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
16
-
5,5'-dithiobis(2-nitrobenzoic acid) full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni
30
-
thioredoxin disulfide full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C Schistosoma mansoni

Cofactor

Cofactor Comment Organism Structure
FAD
-
Schistosoma mansoni
NADPH
-
Schistosoma mansoni