Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Schistosoma mansoni |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00637 | - |
thioredoxin disulfide | full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni | |
0.114 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni | |
0.71 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
SDS-PAGE | Schistosoma mansoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Schistosoma mansoni | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Schistosoma mansoni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 90000, SDS-PAGE | Schistosoma mansoni |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Schistosoma mansoni |
thioredoxin glutathione reductase | chimeric enzyme able to transport reducing equivalents from NADPH to both GSH/GSSG and thioredoxin systems via the fusion of the thioredxin reductase and glutathione reductase domains | Schistosoma mansoni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 100 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni | |
16 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full length enzyme, in 100 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni | |
30 | - |
thioredoxin disulfide | full length enzyme, in 50 mM potassium phosphate pH 7.0, at 25°C | Schistosoma mansoni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Schistosoma mansoni | |
NADPH | - |
Schistosoma mansoni |