Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
Entamoeba histolytica thioredoxin disulfide 41 | pH 7.0, 25°C | Entamoeba histolytica | |
0.0045 | - |
NADPH | pH 7.0, 25°C, cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid) | Entamoeba histolytica | |
0.0046 | - |
Escherichia coli thioredoxin disulfide | pH 7.0, 25°C | Entamoeba histolytica | |
1.7 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | pH 7.0, 25°C | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33700 | - |
x * 33700, calculated from sequence | Entamoeba histolytica |
36000 | - |
x * 36000, SDS-PAGE | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | EhTRXR and EhTRX41 could be assayed as a functional redox pair that, together with peroxiredoxin, mediates the NADPH-dependent reduction of hydrogen peroxide and tert-butyl hydroperoxide. It is proposed that this detoxifying system could be operative in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Entamoeba histolytica | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
Entamoeba histolytica thioredoxin disulfide 41 + NADPH + H+ | - |
Entamoeba histolytica | Entamoeba histolytica thioredoxin 41 + NADP+ | - |
? | |
Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
Entamoeba histolytica | Escherichia coli thioredoxin + NADP+ | - |
? | |
additional information | EhTRXR and EhTRX41 could be assayed as a functional redox pair that, together with peroxiredoxin, mediates the NADPH-dependent reduction of hydrogen peroxide and tert-butyl hydroperoxide. It is proposed that this detoxifying system could be operative in vivo | Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Entamoeba histolytica |
? | x * 33700, calculated from sequence | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
EhTRXR | - |
Entamoeba histolytica |
thioredoxin reductase | - |
Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | calculated from sequence | - |
5.94 |