Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
expression in Escherichia coli | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
hanging drop diffusion method at 20°C, X-ray crystal structure of DmTR at 2.4 A resolution in which the enzyme was truncated to remove the C-terminal tripeptide sequence Cys-Cys-Ser | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH | Mus musculus |
additional information | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence SCCS | Mus musculus | |
0.0676 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
0.123 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCCG | Mus musculus | |
0.141 | - |
thioredoxin disulfide | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
0.17 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
0.47 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
0.92 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid | Drosophila melanogaster | |
2.72 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | DmTR shows high catalytic activity without the presence of a selenocysteine | Drosophila melanogaster | |
additional information | DmTR shows high catalytic activity without the presence of a selenocysteine | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | P91938 | - |
- |
Mus musculus | Q8BTW3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Drosophila melanogaster | thioredoxin + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Mus musculus | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DmTR | - |
Drosophila melanogaster |
mTR3 | - |
Mus musculus |
thioredoxin reductase | - |
Drosophila melanogaster |
thioredoxin reductase | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence SCCS | Mus musculus | |
0.13 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCCG | Mus musculus | |
1.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
2.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid | Drosophila melanogaster | |
5.8 | - |
thioredoxin disulfide | full-length Drosophila enzyme with C-terminal sequence SCCS | Drosophila melanogaster | |
15.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid | Mus musculus | |
20.83 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus | |
37 | - |
thioredoxin disulfide | full-length mouse enzyme with C-terminal sequence GCUG | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Drosophila melanogaster | |
NADPH | - |
Mus musculus |