Literature summary for 1.8.1.9 extracted from

Ruocco, M.R.; Ruggiero, A.; Masullo, L.; Arcari, P.; Masullo, M.
A 35 kDa NAD(P)H oxidase previously isolated from the archaeon Sulfolobus solfataricus is instead a thioredoxin reductase. (2004), Biochimie, 86, 883-892.

Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
C147A	significantly reduced activity, decrease in FAD content	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
auranofin	-	Saccharolobus solfataricus
azelaic acid	-	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0017	-	NADPH	pH 5.5, 60°C	Saccharolobus solfataricus
0.0441	-	thioredoxin	pH 5.5, 60°C	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	2 * 35000, SDS-PAGE	Saccharolobus solfataricus
70000	-	gel filtration	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W27	enzyme shows additionally NADH oxidase activity	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH	-	Saccharolobus solfataricus	2-nitro-5-thiobenzoate + NADP+	-	?
additional information	enzyme shows additionally NADH oxidase activity	Saccharolobus solfataricus	?	-	?
thioredoxin + NADP+	-	Saccharolobus solfataricus	thioredoxin disulfide + NADPH	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.61	-	NADPH	pH 5.5, 60°C	Saccharolobus solfataricus
1.3	-	thioredoxin	pH 5.5, 60°C	Saccharolobus solfataricus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	wild-type, 0.7 mol per mol of subunit, mutant C147A, 0.3 mol per mol of subunit	Saccharolobus solfataricus
NADP+	-	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.013	-	auranofin	pH 5.5, 60°C	Saccharolobus solfataricus
0.054	-	azelaic acid	pH 5.5, 60°C	Saccharolobus solfataricus

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Release 2023.1 (January 2023)