Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
U489C | barely detectable activity towards thioredoxin and hydrogen peroxide | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
thioredoxin | mutant enzyme U489C | Mus musculus | |
6.6 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
35 | - |
thioredoxin | semisynthetic enzyme with 63% content of selenium | Mus musculus | |
67.6 | - |
thioredoxin | semisynthetic enzyme with 91% content of selenium | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JLT4 | - |
- |
Purification (Comment) | Organism |
---|---|
chitin resin column chromatography and DEAE anion exchange column chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Mus musculus |
TR | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
thioredoxin | mutant enzyme U489C | Mus musculus | |
1.183 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
6.08 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
25 | - |
thioredoxin | semisynthetic enzyme with 63% content of selenium | Mus musculus | |
37 | - |
thioredoxin | semisynthetic enzyme with 91% content of selenium | Mus musculus |