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Literature summary for 1.8.1.9 extracted from

  • Tanaka, R.; Kosugi, K.; Mizukami, M.; Ishibashi, M.; Tokunaga, H.; Tokunaga, M.
    Expression and purification of thioredoxin (TrxA) and thioredoxin reductase (TrxB) from Brevibacillus choshinensis (2004), Protein Expr. Purif., 37, 385-391.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with an amino-terminal hexa-His-tag for purification Brevibacillus choshinensis

Organism

Organism UniProt Comment Textmining
Brevibacillus choshinensis Q5KTZ6 formerly Bacillus brevis
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with an amino-terminal hexa-His-tag, extrasequence does not affect enzymatic activity, one-step Ni-NTA affinity column chromatography Brevibacillus choshinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thioredoxin disulfide + NADPH
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Brevibacillus choshinensis thioredoxin + NADP+
-
?

Synonyms

Synonyms Comment Organism
thioredoxin reductase
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Brevibacillus choshinensis
TrxB
-
Brevibacillus choshinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Brevibacillus choshinensis