Any feedback?
Literature summary for 1.8.1.9 extracted from
- Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability (2002), J. Biol. Chem., 277, 11521-11526.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | C-terminal redox active disulfide | Drosophila melanogaster |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of cytosolic and mitochondrial isoform as His-tagged enzymes in Escherichia coli | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | His-tagged transcript specific mutants, varied N-terminus, 1 null-mutant | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
NADPH | cytosolic and mitochondrial TrxR1 isoform | Drosophila melanogaster | |
| 0.007 | - |
thioredoxin | cytosolic TrxR1 isoform | Drosophila melanogaster | |
| 0.007 | - |
thioredoxin-1 disulfide | TrxR(cyto) | Drosophila melanogaster | |
| 0.019 | - |
thioredoxin | mitochondrial TrxR1 isoform | Drosophila melanogaster | |
| 0.019 | - |
thioredoxin-1 disulfide | TRxR(mito) | Drosophila melanogaster | |
| 0.38 | - |
DTNB | cytosolic TrxR1 isoform | Drosophila melanogaster | |
| 0.41 | - |
DTNB | mitochondrial TrxR1 isoform | Drosophila melanogaster | |
| 1 | - |
NADPH | TrxR-1(cyto) | Drosophila melanogaster | |
| 1 | - |
NADPH | TrxR-1(mito) | Drosophila melanogaster | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | TrxR-1(cyto) | Drosophila melanogaster | 5737 | - |
| cytosol | isoform TrxR1 | Drosophila melanogaster | 5829 | - |
| mitochondrion | isoform TrxR3 and TrxR1 | Drosophila melanogaster | 5739 | - |
| mitochondrion | TrxR-1(mito) | Drosophila melanogaster | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | TrxR-1(cyto) is not a selenoprotein | Drosophila melanogaster | |
| additional information | TrxR-1(mito) is not a selenoprotein | Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53200 | - |
cytosolic TrxR1 isoform, deduced from amino acid sequence | Drosophila melanogaster |
| 63700 | - |
mitochondrial TrxR1 isoform, deduced from amino acid sequence | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | Drosophila melanogaster | defense against oxidative stress | thioredoxin disulfide + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Drosophila melanogaster | - |
3 isoforms TrxR1, TrxR2, TrxR3, encoded by 3 genes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type cytosolic and mitochondrial isoforms and mutants from Escherichia coli | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| thioredoxin + NADP+ | coupled assay with DTNB | Drosophila melanogaster | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | defense against oxidative stress | Drosophila melanogaster | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin-1 disulfide + NADPH | - |
Drosophila melanogaster | NADP+ + thioredoxin-1 | - |
? | |
| thioredoxin-1 disulfide + NADPH + H+ | - |
Drosophila melanogaster | thioredoxin-1 + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53200, TrxR-1(cyto), calculation from nucleotide sequence | Drosophila melanogaster |
| ? | x * 63700, TrxR-1(mito), calculation from nucleotide sequence | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrxR | - |
Drosophila melanogaster |
| TrxR-1(cyto) | - |
Drosophila melanogaster |
| TrxR-1(mito) | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
cytosolic and mitochondrial TrxR1 isoform | Drosophila melanogaster |
| 7.1 | - |
TrxR-1(cyto) | Drosophila melanogaster |
| 7.1 | - |
TrxR-1(mito) | Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Drosophila melanogaster | |
| NADPH | - |
Drosophila melanogaster | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Drosophila melanogaster | TrxR-1(cyto) | - |
5.93 |
| Drosophila melanogaster | TrxR-1(mito) | - |
8.15 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
