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Literature summary for 1.8.1.9 extracted from

  • Gromer, S.; Gross, J.H.
    Methylseleninate is a substrate rather than an inhibitor of mammalian thioredoxin reductase: implications for the antitumor effects of selenium (2002), J. Biol. Chem., 277, 9701-9706.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine C-terminal redox active disulfide Drosophila melanogaster
cysteine C-terminal redox active disulfide Homo sapiens
cysteine C-terminal redox active disulfide Plasmodium falciparum
cysteine part of the active site Drosophila melanogaster
cysteine part of the active site Homo sapiens
cysteine part of the active site Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C73S recombinant, His-tagged Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
auranofin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
methylseleninate
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
recombinant
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Plasmodium falciparum
-
recombinant
-

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Mus musculus 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Plasmodium falciparum 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Plasmodium falciparum 2-nitro-5-thiobenzoate + NADP+
-
?
GSSG + NADPH in presence of methylselenol Homo sapiens GSH + NADP+
-
r
methylseleninate + H2O2
-
Drosophila melanogaster ?
-
?
methylseleninate + H2O2
-
Mus musculus ?
-
?
methylseleninate + H2O2
-
Plasmodium falciparum ?
-
?
methylseleninate + H2O2 in presence of methylseleninate Homo sapiens ?
-
?
methylseleninate + NADPH also utilizes glutathione instead of NADPH Homo sapiens CH3SeH + NADP+
-
ir
seleninate + NADPH
-
Homo sapiens ?
-
?
thioredoxin + NADP+
-
Mus musculus thioredoxin disulfide + NADPH
-
r
thioredoxin + NADP+ coupled assay with DTNB Drosophila melanogaster thioredoxin disulfide + NADPH
-
r
thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
r
thioredoxin + NADP+ coupled assay with DTNB Plasmodium falciparum thioredoxin disulfide + NADPH
-
r
thioredoxin + tert-butyl-hydroperoxide in presence of methylseleninate, coupled assay Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TrxR
-
Drosophila melanogaster
TrxR
-
Mus musculus
TrxR
-
Homo sapiens
TrxR
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
methylseleninate recombinant protein Drosophila melanogaster
14
-
methylseleninate
-
Mus musculus
23
-
methylseleninate
-
Homo sapiens
31
-
methylseleninate
-
Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Drosophila melanogaster
FAD
-
Mus musculus
FAD
-
Homo sapiens
FAD
-
Plasmodium falciparum
NADPH
-
Drosophila melanogaster
NADPH
-
Mus musculus
NADPH
-
Homo sapiens
NADPH
-
Plasmodium falciparum