Literature summary for 1.8.1.9 extracted from

Gilberger, T.W.; Walter, R.D.; Muller, S.
Identification and characterization of the functional amino acids at the active site of the large thioredoxin reductase from Plasmodium falciparum (1997), J. Biol. Chem., 272, 29584-29589.

Search for more literature for 1.8.1.9

Activating Compound

Activating Compound	Comment	Organism	Structure
cysteine	at the active center, C88 and C93	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutants in Escherichia coli	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
C88A	site-directed mutagenesis, no activity	Plasmodium falciparum
C88S	site-directed mutagenesis, no activity	Plasmodium falciparum
C93A	site-directed mutagenesis, no activity	Plasmodium falciparum
H509A	site-directed mutagenesis, reduced activity	Plasmodium falciparum
H509Q	site-directed mutagenesis, reduced activity	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	DTNB	mutant enzyme H509Q	Plasmodium falciparum
0.147	-	DTNB	mutant enzyme H509A	Plasmodium falciparum
0.212	-	DTNB	wild-type enzyme	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutants from Escherichia coli	Plasmodium falciparum

Reaction

Reaction	Comment	Organism	Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+	electron transfer in the enzyme complex of apoenzyme, FAD and thioredoxin with NADPH	Plasmodium falciparum	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.22	-	mutant H509Q, coupled assay with DTNB	Plasmodium falciparum
4.3	-	mutant H509A, coupled assay with DTNB	Plasmodium falciparum
4.3	-	wild-type enzyme, coupled assay with DTNB	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH	i.e. DTNB	Plasmodium falciparum	2-nitro-5-thiobenzoate + NADP+	-	?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH	coupled assay	Plasmodium falciparum	2-nitro-5-thiobenzoate + NADP+	-	?
thioredoxin + NADP+	coupled assay with DTNB	Plasmodium falciparum	thioredoxin disulfide + NADPH	-	?
thioredoxin + NADP+	coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin	Plasmodium falciparum	thioredoxin disulfide + NADPH	-	?

Synonyms

Synonyms	Comment	Organism
More	2 classes of thioredoxin reductases: high molecular weight or large proteins e.g. in mammalia, Plasmodium falsiparum and Caenorhabditis elegans, and low molecular weight proteins e.g. in bacteria	Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.16	-	DTNB	mutant enzyme H509A	Plasmodium falciparum
0.233	-	DTNB	mutant enzyme H509Q	Plasmodium falciparum
4.58	-	DTNB	wild-type enzyme	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Plasmodium falciparum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Plasmodium falciparum
NADPH	-	Plasmodium falciparum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)