Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.9 extracted from

  • Tamura, T.; Stadtman, T.C.
    A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity (1996), Proc. Natl. Acad. Sci. USA, 93, 1006-1011.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
3-Bromopropionate
-
Homo sapiens
arsenite
-
Homo sapiens
iodoacetate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
thioredoxin thioredoxin from rat Homo sapiens
0.034
-
thioredoxin thioredoxin from E. coli Homo sapiens
0.116
-
DTNB
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
selenocysteine contains selenocysteine as part of the redox active selenosulfide in the active center Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
2 * 57000, SDS-PAGE Homo sapiens
110000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycosyl groups on the protein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lung adenocarcinoma cell line NCI-H441 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
DTNB coupled assay, purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
GSSG + NADPH
-
Homo sapiens GSH + NADP+
-
?
menadione + NADPH
-
Homo sapiens ?
-
?
oxidized lipoate + NADPH
-
Homo sapiens ?
-
?
rat thioredoxin + NADP+
-
Homo sapiens rat thioredoxin disulfide + NADPH + H+
-
r
thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Homo sapiens thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+
-
Homo sapiens thioredoxin disulfide + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 57000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.83
-
rat thioredoxin insulin coupled assay Homo sapiens
5.58
-
thioredoxin insulin coupled assay method Homo sapiens
29.5
-
DTNB
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
NADPH
-
Homo sapiens