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Literature summary for 1.8.1.9 extracted from
- Cloning of thioredoxin h reductase and characterization of the thioredoxin reductase-thioredoxin h system from wheat (2002), Biochem. J., 367, 491-497.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Triticum aestivum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a His-tagged protein | Triticum aestivum |
| expression of wild-type as His-tagged protein and mutant C53S in Escherichia coli, DNA sequence analysis | Triticum aestivum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C53S | site-directed mutagenesis, no activity since the redox cycle system is abolished | Triticum aestivum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
NADPH | - |
Triticum aestivum | |
| 0.0004 | - |
NADPH | wild-type | Triticum aestivum | |
| 0.00076 | - |
thioredoxin | assay with DTNB instead of NADPH | Triticum aestivum | |
| 0.0076 | - |
thioredoxin disulfide h | - |
Triticum aestivum | |
| 0.036 | - |
thioredoxin | wild-type, thioredoxin from E. coli | Triticum aestivum | |
| 0.6 | - |
NADH | - |
Triticum aestivum | |
| 0.6 | - |
NADH | wild-type | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | Q8VX47 | - |
- |
| Triticum aestivum | Q8VX47 | cv. Chineses Spring | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant, wild-type and mutant C53S | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Triticum aestivum | - |
| leaf | from mature plants, high accumulation | Triticum aestivum | - |
| root | from 2-4-day-old seedlings, high accumulation | Triticum aestivum | - |
| seed | aleurone layer, scutellum, starchy endosperm | Triticum aestivum | - |
| seed | low accumulation | Triticum aestivum | - |
| seedling | - |
Triticum aestivum | - |
| seedling | root and shoot | Triticum aestivum | - |
| shoot | from 2-4-day-old seedlings, high accumulation | Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Triticum aestivum | ? | - |
? | |
| thioredoxin + NADP+ | coupled assay with DTNB | Triticum aestivum | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Triticum aestivum | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | in presence of NADPH, coupled assay | Triticum aestivum | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | i.e. DTNB | Triticum aestivum | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin disulfide h + NADH | - |
Triticum aestivum | thioredoxin + NAD+ | - |
? | |
| thioredoxin disulfide h + NADPH | - |
Triticum aestivum | thioredoxin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTR | - |
Triticum aestivum |
| thioredoxin h reductase | - |
Triticum aestivum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Triticum aestivum | |
| NADPH | specific for | Triticum aestivum | |
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