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Literature summary for 1.8.1.9 extracted from
- Rat liver thioredoxin and thioredoxin reductase: purification and characterization (1982), Biochemistry, 21, 6628-6633.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | part of the active site | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
thioredoxin | substrate: thioredoxin from calf and rat | Rattus norvegicus | |
| 0.0025 | - |
reductase | thioredoxin from rat, bovine substrate | Rattus norvegicus | |
| 0.006 | - |
NADPH | - |
Rattus norvegicus | |
| 0.045 | - |
thioredoxin | substrate: thioredoxin from E. coli | Rattus norvegicus | |
| 0.66 | - |
DTNB | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
| 116000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | - |
purified enzyme, coupled assay with DTNB | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| alloxan + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| L-cysteine + NADPH | - |
Rattus norvegicus | L-cystine + NADP+ | - |
? | |
| menadione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| oxidized lipoamide + NADPH | - |
Rattus norvegicus | lipoamide disulfide + NADP+ | - |
? | |
| thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH | - |
ir | |
| thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
thioredoxin | - |
Rattus norvegicus | |
| 66.7 | - |
DTNB | - |
Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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