Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.9 extracted from

  • Clement-Metral, J.D.; Höög, J.O.; Holmgren, A.
    Characterization of the thioredoxin system in the facultative phototroph Rhodobacter sphaeroides Y [published erratum appears in Eur J Biochem 1987 May 4;164(3):713] (1986), Eur. J. Biochem., 161, 119-126.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine part of the active site Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
thioredoxin
-
Cereibacter sphaeroides
0.0033
-
reductase
-
Cereibacter sphaeroides
0.0144
-
thioredoxin substrate: thioredoxin from E. coli Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Cereibacter sphaeroides
68000
-
native PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
Y
-
Cereibacter sphaeroides Y
-
Y
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Cereibacter sphaeroides 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH requires thioredoxin for reduction of DTNB Cereibacter sphaeroides 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Cereibacter sphaeroides Y 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH requires thioredoxin for reduction of DTNB Cereibacter sphaeroides Y 2-nitro-5-thiobenzoate + NADP+
-
?
additional information
-
Cereibacter sphaeroides ?
-
?
additional information
-
Cereibacter sphaeroides Y ?
-
?
thioredoxin + NADP+
-
Cereibacter sphaeroides thioredoxin disulfide + NADPH
-
r
thioredoxin + NADP+
-
Cereibacter sphaeroides Y thioredoxin disulfide + NADPH
-
r
thioredoxin + NADP+ in presence of NADPH, coupled assay Cereibacter sphaeroides thioredoxin disulfide + NADPH + H+
-
r
thioredoxin + NADP+ in presence of NADPH, coupled assay Cereibacter sphaeroides Y thioredoxin disulfide + NADPH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
TRase
-
Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
FAD 2 FAD per dimer Cereibacter sphaeroides
NADPH strictly dependent on NADPH, inactive with NADH Cereibacter sphaeroides