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Literature summary for 1.8.1.7 extracted from

  • Marty, L.; Siala, W.; Schwarzlaender, M.; Fricker, M.D.; Wirtz, M.; Sweetlove, L.J.; Meyer, Y.; Meyer, A.J.; Reichheld, J.P.; Hell, R.
    The NADPH-dependent thioredoxin system constitutes a functional backup for cytosolic glutathione reductase in Arabidopsis (2009), Proc. Natl. Acad. Sci. USA, 106, 9109-9114.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1,3-Bis(2-chloroethyl)-1-nitrosourea significant inhibition of recombinant isoform GR1 is observed at high concentrations of 1 mM and above Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 NADPH in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 Arabidopsis thaliana
0.077
-
 glutathione disulfide in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isoform GR2 Arabidopsis thaliana 9507
-
cytosol isoform GR1 Arabidopsis thaliana 5829
-
mitochondrion isoform GR2 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57100
-
 2 * 57100, calculated for GR1 including the N-terminal His-tag Arabidopsis thaliana
60000
-
 2 * 60000, SDS-PAGE Arabidopsis thaliana
120000
-
 purified native isoform GR1, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
 Arabidopsis thaliana glutathione + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Arabidopsis thaliana
homodimer 2 * 57100, calculated for GR1 including the N-terminal His-tag Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
glutathione reductase
-
 Arabidopsis thaliana
GR1 cytosolic isoform, about 65% of the total glutathione reductase activity is attributed to isoform GR1 Arabidopsis thaliana
GR2 isoform dual-targeted to chloroplasts and mitochondria, about 35% of the total glutathione reductase activity is attributed to isoform GR2 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.6
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction deletion of isoform GR1 prevents survival due to a pollen lethal phenotype Arabidopsis thaliana