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Literature summary for 1.8.1.7 extracted from

  • Cardoso, L.; Ferreira, S.; Hermes-Lima, M.
    Reductive inactivation of yeast glutathione reductase by Fe(II) and NADPH (2008), Comp. Biochem. Physiol. A, 151, 313-321.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ addition of exogenous Fe2+ (but not Fe3+) potentiates NADPH-induced inactivation of glutathione reductase, after a 2 min incubation period, 0.05 mM Fe2+ plus 0.3 mM NADPH induce 57% loss of enzyme activity Saccharomyces cerevisiae
NADPH 58% inhibition at 0.3 mM after 60 min incubation, exogenously added antioxidants including ethanol, dimethylsulfoxide and 2-deoxyribose do not protect glutathione reductase against NADPH-induced inactivation, whilst addition of exogenous Fe2+ (but not Fe3+) potentiates the inactivation, removal of oxygen from the medium leads to increased inhibition of glutathione reductase, whereas pre-incubation of the Fe2+-containing medium for 30 min under normoxic conditions prior to the addition of GR abolishes the enzyme inactivation by NADPH Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
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Saccharomyces cerevisiae glutathione + NADP+
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Synonyms

Synonyms Comment Organism
glutathione reductase
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Saccharomyces cerevisiae
NADPH
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Saccharomyces cerevisiae