Literature summary for 1.8.1.7 extracted from

Willmore, W.G.; Storey, K.B.
Purification and properties of glutathione reductase from liver of the anoxia-tolerant turtle, Trachemys scripta elegans (2007), Mol. Cell. Biochem., 297, 139-149.

Search for more literature for 1.8.1.7

Inhibitors

Inhibitors	Comment	Organism	Structure
glutathione	inhibits the turtle liver GR only at high concentrations (6 mM), although this I50 value is within the physiological range of GSH concentrations in most cell types	Trachemys scripta
KCl	IC50: 0.51 M	Trachemys scripta
additional information	NADP+ does not inhibit turtle GR activity	Trachemys scripta
Na2SO4	IC50: 0.45 M	Trachemys scripta
NaCl	IC50: 0.55 M	Trachemys scripta
NH4Cl	IC50: 0.62 M	Trachemys scripta
Sodium acetate	IC50: 0.77 M	Trachemys scripta

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00446	-	GSSG	anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH	Trachemys scripta
0.00682	-	NADPH	anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH	Trachemys scripta

Metals/Ions

Metals/Ions	Comment	Organism	Structure
KCl	IC50: 0.51 M	Trachemys scripta

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE	Trachemys scripta

Organism

Organism	UniProt	Comment	Textmining
Trachemys scripta	-	Trachemys scripta elegans	-

Purification (Commentary)

Purification (Comment)	Organism
ion exchange chromatography on hydroxylapatite, LKB 8101 isoelectrofocusing column chromatography, Sephacryl S-300 column gel filtration and Matrix Red dye ligand chromatography	Trachemys scripta

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Trachemys scripta	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.119	-	crude supernatant of anoxic enzyme	Trachemys scripta
0.418	-	crude supernatant of aerobic enzyme	Trachemys scripta
1.44	-	anoxic enzyme after 109fold purification	Trachemys scripta
5.5	-	aerobic enzyme after 109fold purification	Trachemys scripta

Storage Stability

Storage Stability	Organism
4°C, pure enzyme without stabilizers such as glycerol, 8 days, 25% loss of activity	Trachemys scripta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GSSG + NADPH + H+	-	Trachemys scripta	glutathione + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
glutathione reductase	-	Trachemys scripta

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Trachemys scripta

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	10	-	Trachemys scripta

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Trachemys scripta

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trachemys scripta	aerobic enzyme	-	7.2
Trachemys scripta	anoxic enzyme	-	7.32

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
450	-	IC50: 0.45 M	Trachemys scripta	Na2SO4
510	-	IC50: 0.51 M	Trachemys scripta	KCl
550	-	IC50: 0.55 M	Trachemys scripta	NaCl
620	-	IC50: 0.62 M	Trachemys scripta	NH4Cl
770	-	IC50: 0.77 M	Trachemys scripta	Sodium acetate

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Release 2023.1 (January 2023)