Crystallization (Comment) | Organism |
---|---|
peroxynitrite inactivated enzyme, hanging drop vapour dffusion method, 10 mg/ml protein in 120 mM ammonium sulfate, 100 mM potassium phosphate, pH 7.5, against reservoir solution containing 720 mM ammonium sulfate, 100 mM potassium phosphate, pH 8.0, 30-37°C, 5 days, storage and handling in stabilization solution containing 1 M ammonium sulfate, 100 mM potassium phosphate, pH 8.0, X-ray structure determination and analysis at 1.9 A resolution | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
Y114L | site-directed mutagenesis, mutant is insensitive to inactivation by peroxynitrite in contrast to the wild-type enzyme | Homo sapiens |
Y114L | site-directed mutagenesis, mutant is insensitive to inactivation by peroxynitrite in contratst to the wild-type enzyme | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | bis-nitro-enzyme form is impaired in peptide substrate binding with a 20fold increased Km for glutathione disulfide, overview | Homo sapiens | |
peroxynitrite | inactivation by modification of Tyr114 and Tyr106 | Homo sapiens | |
peroxynitrite | inactivation by modification of Tyr114 and Tyr106 | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
glutathione disulfide | pH 7.4, 25°C | Homo sapiens | |
0.095 | - |
glutathione disulfide | pH 7.4, 25°C | Plasmodium falciparum | |
1.65 | - |
glutathione disulfide | mutant enzyme, pH 7.4, 25°C | Homo sapiens | |
2.31 | - |
glutathione disulfide | mutant enzyme, pH 7.4, 25°C | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | Plasmodium falciparum | - |
glutathione + NADP+ | - |
? | |
glutathione disulfide + NADPH | Homo sapiens | - |
glutathione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00390 | - |
- |
Plasmodium falciparum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Plasmodium falciparum |
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | - |
Plasmodium falciparum | glutathione + NADP+ | - |
? | |
glutathione disulfide + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
glutathione disulfide + NADPH | enzyme contains an active site disulfide/dithiol depending on the redox status | Plasmodium falciparum | glutathione + NADP+ | - |
? | |
glutathione disulfide + NADPH | enzyme contains an active site disulfide/dithiol depending on the redox status | Homo sapiens | glutathione + NADP+ | - |
? | |
peroxynitrite + NADPH | - |
Plasmodium falciparum | nitrite + H2O + NADP+ | - |
? | |
peroxynitrite + NADPH | - |
Homo sapiens | nitrite + H2O + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Plasmodium falciparum |
glutathione reductase | - |
Homo sapiens |
GR | - |
Plasmodium falciparum |
GR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
glutathione disulfide | mutant enzyme, pH 7.4, 25°C | Plasmodium falciparum | |
5 | - |
glutathione disulfide | mutant enzyme, pH 7.4, 25°C | Homo sapiens | |
120 | - |
glutathione disulfide | pH 7.4, 25°C | Plasmodium falciparum | |
165 | - |
glutathione disulfide | pH 7.4, 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Plasmodium falciparum |
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Plasmodium falciparum | |
NADPH | - |
Homo sapiens |