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Literature summary for 1.8.1.7 extracted from
- Oxidative stress response in yeast: purification and characterization of glutathione reductase from Hansenula mrakii (1996), Biosci. Biotechnol. Biochem., 60, 1207-1209.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Cyberlindnera mrakii |  |
| Co2+ | slightly | Cyberlindnera mrakii | |
| Cu2+ | - |
Cyberlindnera mrakii | |
| Fe3+ | - |
Cyberlindnera mrakii | |
| Ni2+ | slightly | Cyberlindnera mrakii | |
| p-chloromercuribenzoate | - |
Cyberlindnera mrakii | |
| Zn2+ | - |
Cyberlindnera mrakii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0143 | - |
NADPH | - |
Cyberlindnera mrakii | |
| 0.0213 | - |
GSSG | - |
Cyberlindnera mrakii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight activation | Cyberlindnera mrakii | |
| Mn2+ | activates | Cyberlindnera mrakii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, SDS-PAGE | Cyberlindnera mrakii |
| 123000 | - |
gel filtration | Cyberlindnera mrakii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera mrakii | - |
yeast, IFO 0895 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera mrakii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 86.9 | - |
purified enzyme | Cyberlindnera mrakii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Cyberlindnera mrakii | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, SDS-PAGE | Cyberlindnera mrakii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GR | - |
Cyberlindnera mrakii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Cyberlindnera mrakii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cyberlindnera mrakii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
NADPH + GSSG | Cyberlindnera mrakii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Cyberlindnera mrakii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Cyberlindnera mrakii | |
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Release 2023.1 (January 2023)
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