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Literature summary for 1.8.1.7 extracted from

  • Takeda, T.; Ishikawa, T.; Shigeoka, S.; Hirayama, O.; Mitsunaga, T.
    Purification and characterization of glutathione reductase from Chlamydomonas reinhardtii (1993), J. Gen. Microbiol., 139, 2233-2238.
No PubMed abstract available
Search for more literature for 1.8.1.7

Inhibitors

Inhibitors Comment Organism Structure
Co2+ complete inhibition at 1 mM Chlamydomonas reinhardtii
Cu2+ complete inhibition at 1 mM Chlamydomonas reinhardtii
Hg2+ complete inhibition at 1 mM Chlamydomonas reinhardtii
Mn2+ 40% inhibition Chlamydomonas reinhardtii
N-ethylmaleimide
-
 Chlamydomonas reinhardtii
Ni2+ 80% inhibition Chlamydomonas reinhardtii
p-chloromercuribenzoate
-
 Chlamydomonas reinhardtii
Zn2+ 75% inhibition Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
 NADPH
-
 Chlamydomonas reinhardtii
0.054
-
 GSSG
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000 56000 gel filtration Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii

Reaction

Reaction Comment Organism Reaction ID
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ branching mechanism Chlamydomonas reinhardtii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
373.3
-
 purified enzyme Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSSG + NADPH highly specific for GSSG Chlamydomonas reinhardtii glutathione + NADP+
-
 ir

Subunits

Subunits Comment Organism
monomer 1 * 54000-56000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
49
-
-
 Chlamydomonas reinhardtii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
31 54 49% activity at 31°C and 68% activity at 54°C Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable Chlamydomonas reinhardtii
55
-
 complete loss of activity Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.4
-
 Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
FAD FAD enzyme Chlamydomonas reinhardtii
additional information no activity with FMN Chlamydomonas reinhardtii
NADPH absolute specificity for NADPH Chlamydomonas reinhardtii