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Literature summary for 1.8.1.7 extracted from
- Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography (1978), Planta, 139, 9-17.
General Stability
| General Stability | Organism |
|---|---|
| complete inactivation after incubation with NADPH, reversible by addition of GSSG | Spinacia oleracea |
| glutathione stabilizes chloroplast enzymes | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | product inhibition | Spinacia oleracea |  |
| iodoacetate | - |
Spinacia oleracea | |
| N-ethylmaleimide | - |
Spinacia oleracea | |
| NADP+ | competitive against NADPH | Spinacia oleracea | |
| Zn2+ | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
NADPH | chloroplast enzyme | Spinacia oleracea | |
| 0.21 | - |
GSSG | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
2 * 72000, SDS-PAGE | Spinacia oleracea |
| 145000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 246 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, several weeks | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | reverse reaction only if very high concentration of NADP+ and glutathione present | Spinacia oleracea | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 72000, SDS-PAGE | Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, complete loss of activity | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7: about 40% of activity maximum, pH 9.5: about 55% of activity maximum | Spinacia oleracea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
30 min, stable | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | low activity with NADH | Spinacia oleracea | |
| NADPH | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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