General Stability | Organism |
---|---|
complete inactivation after incubation with NADH, reversible by addition of GSSG | Mus musculus |
complete inactivation after incubation with NADPH, reversible by addition of GSSG | Mus musculus |
dichloroindophenol protects against thermal inactivation at 80°C | Mus musculus |
FAD, no stabilization against thermal inactivation at 80°C | Mus musculus |
glutathione protects against thermal inactivation at 80°C | Mus musculus |
GSSG protects against thermal inactivation at 80°C | Mus musculus |
NADP+, stabilizes against thermal inactivation at 80°C | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | product inhibition | Mus musculus | |
NADH | at concentration above 0.3 mM | Mus musculus | |
NADP+ | competitive against NADPH | Mus musculus | |
NADPH | at concentration above 0.3 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0059 | - |
NADPH | - |
Mus musculus | |
0.107 | - |
GSSG | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Mus musculus |
105000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-70°C, pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 0.020 mM FAD, 5 mM NADP+ | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Mus musculus | glutathione + NADP+ | - |
? | |
additional information | branched mechanism | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
8 h, stable | Mus musculus |
75 | - |
10 min, stable | Mus musculus |
80 | - |
NADP+, GSSG, glutathione and dichloroindophenol protect against thermal inactivation at 80°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |