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Literature summary for 1.8.1.7 extracted from

  • Bielawski, W.; Joy, K.W.
    Properties of glutathione reductase from chloroplasts and roots of pea (1986), Phytochemistry, 25, 2261-2265.
No PubMed abstract available
Search for more literature for 1.8.1.7

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ root enzyme inhibited, chloroplast enzyme only slightly Pisum sativum
Fe2+ root enzyme inhibited, chloroplast enzyme only slightly Pisum sativum
N-ethylmaleimide root enzyme is slightly more sensitive than chloroplast enzyme Pisum sativum
p-hydroxymercuribenzoate chloroplast enzyme is slightly more sensitive than root enzyme Pisum sativum
Zn2+ root enzyme inhibited, chloroplast enzyme very slightly Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 NADPH root enzyme Pisum sativum
0.003
-
 NADPH chloroplast enzyme Pisum sativum
0.01
-
 GSSG root enzyme Pisum sativum
0.028
-
 GSSG chloroplast enzyme Pisum sativum
0.22
-
 NADP+ root and chloroplast enzyme Pisum sativum
7.7
-
 glutathione chloroplast enzyme Pisum sativum
8.2
-
 glutathione root enzyme Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Pisum sativum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
156000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 pea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-
root
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
 purified enzyme Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSSG + NADPH
-
 Pisum sativum glutathione + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Pisum sativum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 root enzyme more stable than chloroplast enzyme, oxidized form more stable than reduced form Pisum sativum
30
-
 reduced form of the enzyme highly unstable Pisum sativum
60 65 oxidized form stable Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 root enzyme Pisum sativum
8.2
-
 chloroplast enzyme Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
6.1 9.1 50% of maximal activity at pH 6.1 and 9.1, root enzyme Pisum sativum
6.9 9.2 pH 6.9: about 50% of activity maximum, pH 9.2: about 83% of activity maximum Pisum sativum

Cofactor

Cofactor Comment Organism Structure
NADH 20times higher activity with NADH than with NADPH Pisum sativum
NADPH 5% of the activity with NADH, 3fold higher affinity of root enzyme compared to chloroplast enzyme Pisum sativum