Any feedback?
Literature summary for 1.8.1.7 extracted from
- Purification and characterization of glutathione reductase from mycelium of Phycomyces blakesleeanus (1988), Biochim. Biophys. Acta, 952, 56-66.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| urea, quite stable to | Phycomyces blakesleeanus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Phycomyces blakesleeanus |  |
| NADPH | - |
Phycomyces blakesleeanus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
NADPH | - |
Phycomyces blakesleeanus | |
| 0.13 | - |
GSSG | - |
Phycomyces blakesleeanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50200 | - |
2 * 50200 | Phycomyces blakesleeanus |
| 100000 | - |
- |
Phycomyces blakesleeanus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | Phycomyces blakesleeanus | - |
glutathione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phycomyces blakesleeanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phycomyces blakesleeanus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Phycomyces blakesleeanus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.2 | - |
- |
Phycomyces blakesleeanus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.5, 50 mM sodium phosphate buffer, 1 mM 2-mercaptoethanol, 1 mM EDTA | Phycomyces blakesleeanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Phycomyces blakesleeanus | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50200 | Phycomyces blakesleeanus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42.5 | - |
complete loss of activity above 42°C, 10 min in presence of NADPH | Phycomyces blakesleeanus |
| 55 | - |
10 min, 13% loss of activity | Phycomyces blakesleeanus |
| 65 | - |
10 min, complete loss of activity | Phycomyces blakesleeanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
NADH | Phycomyces blakesleeanus |
| 7.5 | - |
NADPH + GSSG | Phycomyces blakesleeanus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2 mol of FAD per mol of enzyme | Phycomyces blakesleeanus | |
| NADPH | - |
Phycomyces blakesleeanus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
