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Literature summary for 1.8.1.4 extracted from

  • Stibingerova, A.; Velvarska, H.; Kynclova, K.; Marounkova, B.; Spundova, M.; Entlicher, G.
    Lipoamide dehydrogenase and diaphorase catalyzed conversion of some NO donors to NO and reduction of NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) (2009), Gen. Physiol. Biophys., 28, 384-390.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diphenyleneiodonium chloride
-
Bos taurus
diphenyleneiodonium chloride
-
Sus scrofa
additional information LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate Bos taurus
additional information LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
S-nitrosoglutathione in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.42
-
S-nitroso-N-acetylpenicillamine in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.44
-
S-nitrosoglutathione in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.46
-
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
0.84
-
sodium nitroprusside in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.9
-
glycerol trinitrate in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.96
-
acetaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.42
-
glycerol trinitrate in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
1.45
-
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
2.26
-
sodium nitroprusside in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
2.27
-
glycerol trinitrate in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
2.73
-
formaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
3.24
-
Hydroxylamine hydrochloride in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
5.48
-
S-nitroso-N-acetylpenicillamine in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
15.83
-
formaldoxime in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
16.93
-
glycerol trinitrate in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
17.18
-
formaldoxime in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
17.82
-
formaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-
intestine
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH
-
Sus scrofa ?
-
?
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH
-
Bos taurus ?
-
?
acetaldoxime + NADH
-
Sus scrofa ?
-
?
acetaldoxime + NADH
-
Bos taurus ?
-
?
dihydrolipoamide + NAD+
-
Sus scrofa lipoamide + NADH
-
?
dihydrolipoamide + NAD+
-
Bos taurus lipoamide + NADH
-
?
formaldoxime + NADH
-
Sus scrofa ?
-
?
formaldoxime + NADH
-
Bos taurus ?
-
?
glycerol trinitrate + NADH
-
Sus scrofa ?
-
?
glycerol trinitrate + NADH
-
Bos taurus ?
-
?
hydroxylamine hydrochloride + NADH
-
Sus scrofa ?
-
?
hydroxylamine hydrochloride + NADH
-
Bos taurus ?
-
?
additional information LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine Sus scrofa ?
-
?
additional information LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine Bos taurus ?
-
?
S-nitroso-N-acetylpenicillamine + NADH
-
Sus scrofa ?
-
?
S-nitroso-N-acetylpenicillamine + NADH
-
Bos taurus ?
-
?
S-nitrosoglutathione + NADH
-
Sus scrofa ?
-
?
S-nitrosoglutathione + NADH
-
Bos taurus ?
-
?
sodium nitroprusside + NADH
-
Sus scrofa ?
-
?
sodium nitroprusside + NADH
-
Bos taurus ?
-
?

Synonyms

Synonyms Comment Organism
LAD
-
Sus scrofa
LAD
-
Bos taurus
lipoamide dehydrogenase
-
Sus scrofa
lipoamide dehydrogenase
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sus scrofa
FAD
-
Bos taurus
NADH
-
Sus scrofa
NADH
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.29
-
diphenyleneiodonium chloride using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.31
-
diphenyleneiodonium chloride using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.35
-
diphenyleneiodonium chloride using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.45
-
diphenyleneiodonium chloride using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.56
-
diphenyleneiodonium chloride using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.6
-
diphenyleneiodonium chloride using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
0.8
-
diphenyleneiodonium chloride using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.59
-
using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
0.68
-
using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.75
-
using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.81
-
using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.93
-
using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
2.61
-
using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
3.31
-
using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride