Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphenyleneiodonium chloride | - |
Bos taurus | |
diphenyleneiodonium chloride | - |
Sus scrofa | |
additional information | LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate | Bos taurus | |
additional information | LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
S-nitrosoglutathione | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.42 | - |
S-nitroso-N-acetylpenicillamine | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.44 | - |
S-nitrosoglutathione | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.46 | - |
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Bos taurus | |
0.84 | - |
sodium nitroprusside | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.9 | - |
glycerol trinitrate | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.96 | - |
acetaldoxime | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
1.42 | - |
glycerol trinitrate | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Bos taurus | |
1.45 | - |
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
2.26 | - |
sodium nitroprusside | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
2.27 | - |
glycerol trinitrate | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
2.73 | - |
formaldoxime | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
3.24 | - |
Hydroxylamine hydrochloride | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
5.48 | - |
S-nitroso-N-acetylpenicillamine | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
15.83 | - |
formaldoxime | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Bos taurus | |
16.93 | - |
glycerol trinitrate | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Bos taurus | |
17.18 | - |
formaldoxime | in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
17.82 | - |
formaldoxime | in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
intestine | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH | - |
Sus scrofa | ? | - |
? | |
2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH | - |
Bos taurus | ? | - |
? | |
acetaldoxime + NADH | - |
Sus scrofa | ? | - |
? | |
acetaldoxime + NADH | - |
Bos taurus | ? | - |
? | |
dihydrolipoamide + NAD+ | - |
Sus scrofa | lipoamide + NADH | - |
? | |
dihydrolipoamide + NAD+ | - |
Bos taurus | lipoamide + NADH | - |
? | |
formaldoxime + NADH | - |
Sus scrofa | ? | - |
? | |
formaldoxime + NADH | - |
Bos taurus | ? | - |
? | |
glycerol trinitrate + NADH | - |
Sus scrofa | ? | - |
? | |
glycerol trinitrate + NADH | - |
Bos taurus | ? | - |
? | |
hydroxylamine hydrochloride + NADH | - |
Sus scrofa | ? | - |
? | |
hydroxylamine hydrochloride + NADH | - |
Bos taurus | ? | - |
? | |
additional information | LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine | Sus scrofa | ? | - |
? | |
additional information | LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine | Bos taurus | ? | - |
? | |
S-nitroso-N-acetylpenicillamine + NADH | - |
Sus scrofa | ? | - |
? | |
S-nitroso-N-acetylpenicillamine + NADH | - |
Bos taurus | ? | - |
? | |
S-nitrosoglutathione + NADH | - |
Sus scrofa | ? | - |
? | |
S-nitrosoglutathione + NADH | - |
Bos taurus | ? | - |
? | |
sodium nitroprusside + NADH | - |
Sus scrofa | ? | - |
? | |
sodium nitroprusside + NADH | - |
Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAD | - |
Sus scrofa |
LAD | - |
Bos taurus |
lipoamide dehydrogenase | - |
Sus scrofa |
lipoamide dehydrogenase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sus scrofa | |
FAD | - |
Bos taurus | |
NADH | - |
Sus scrofa | |
NADH | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
diphenyleneiodonium chloride | using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.31 | - |
diphenyleneiodonium chloride | using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.35 | - |
diphenyleneiodonium chloride | using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.45 | - |
diphenyleneiodonium chloride | using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.56 | - |
diphenyleneiodonium chloride | using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.6 | - |
diphenyleneiodonium chloride | using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | |
0.8 | - |
diphenyleneiodonium chloride | using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.59 | - |
using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
0.68 | - |
using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
1.75 | - |
using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
1.81 | - |
using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
1.93 | - |
using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
2.61 | - |
using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride | |
3.31 | - |
using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C | Sus scrofa | diphenyleneiodonium chloride |