Literature summary for 1.8.1.4 extracted from

Tyagi, T.K.; Ponnan, P.; Singh, P.; Bansal, S.; Batra, A.; Collin, F.; Guillonneau, F.; Jore, D.; Patkar, S.A.; Saxena, R.K.; Parmar, V.S.; Rastogi, R.C.; Raj, H.G.
Moonlighting protein in Starkeyomyces koorchalomoides: characterization of dihydrolipoamide dehydrogenase as a protein acetyltransferase utilizing acetoxycoumarin as the acetyl group donor (2009), Biochimie, 91, 868-875.

Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Starkeyomyces koorchalomoides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.63	-	alpha-lipoamide	in 0.1 mM Tris-HCl (pH 7.0), 0.0005 mM EDTA, 0.01 mM beta2-mercaptoethanol, at 37°C	Starkeyomyces koorchalomoides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	mitochondrial matrix	Starkeyomyces koorchalomoides	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	SDS-PAGE	Starkeyomyces koorchalomoides

Organism

Organism	UniProt	Comment	Textmining
Starkeyomyces koorchalomoides	-	-	-
Starkeyomyces koorchalomoides FDUS 0337	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Ni-NTA agarose column chromatography	Starkeyomyces koorchalomoides

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Starkeyomyces koorchalomoides	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-lipoamide + NADH	-	Starkeyomyces koorchalomoides	dihydrolipoamide + NAD+	-	?
alpha-lipoamide + NADH	-	Starkeyomyces koorchalomoides FDUS 0337	dihydrolipoamide + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
dihydrolipoamide dehydrogenase	-	Starkeyomyces koorchalomoides
LADH	LADH is an E3 component of pyruvate dehydrogenase complex with significant protein acetyltransferase activity	Starkeyomyces koorchalomoides
TAase	Starkeyomyces koorchalomoides transacetylase (TAase) is a dihydrolipoamide dehydrogenase and also exhibits diaphorase activity	Starkeyomyces koorchalomoides

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Starkeyomyces koorchalomoides
NADH	-	Starkeyomyces koorchalomoides

General Information

General Information	Comment	Organism
physiological function	the protein acetyltransferase activity of LADH can be attributed as a moonlighting function of the enzyme	Starkeyomyces koorchalomoides

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Release 2023.1 (January 2023)