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Literature summary for 1.8.1.4 extracted from

  • Wang, Y.C.; Wang, S.T.; Li, C.; Liu, W.H.; Chen, P.R.; Chen, L.Y.; Liu, T.C.
    The role of N286 and D320 in the reaction mechanism of human dihydrolipoamide dehydrogenase (E3) center domain (2007), J. Biomed. Sci., 14, 203-210.
    View publication on PubMed
Search for more literature for 1.8.1.4

Application

Application Comment Organism
degradation N286 and D320 play a role in the catalytic function of the E3 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens
His-tagged wild-type and mutant proteins overexpressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D320N 48.60% specific activity of the wild type enzyme, 82.7% of FAD content compared to that of the wild-type enzyme Homo sapiens
D320N specific activity is 48.6% to that of the wild-type E3. About 82.7% of FAD content compared to that of wild-type E3. Forms the dimer Homo sapiens
N286D 30.84% specific activity of the wild type enzyme, 96% of FAD content compared to that of the wild-type enzyme Homo sapiens
N286D specific activity is 30.84% to that of the wild-type E3. About 96.0% of FAD content compared to that of wild-type E3. Forms the dimer Homo sapiens
N286Q 24.57% specific activity of the wild type enzyme, 99.4% of FAD content compared to that of the wild-type enzyme Homo sapiens
N286Q specific activity is 24.57% to that of the wild-type E3. About 99.4% of FAD content compared to that of wild-type E3. Forms the dimer Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADH wild-type Homo sapiens
0.04
-
 NADH wild type enzyme Homo sapiens
0.05
-
 Lipoamide mutant enzyme N286Q Homo sapiens
0.05
-
 Lipoamide mutant N286Q Homo sapiens
0.07
-
 Lipoamide mutant enzyme N286D Homo sapiens
0.07
-
 Lipoamide mutant N286D Homo sapiens
0.13
-
 NADH mutant enzyme N286D Homo sapiens
0.13
-
 NADH mutant N286D Homo sapiens
0.14
-
 NADH mutant enzyme N286Q Homo sapiens
0.14
-
 NADH mutant N286Q Homo sapiens
0.16
-
 NAD+ mutant enzyme N286Q Homo sapiens
0.16
-
 NAD+ mutant N286Q Homo sapiens
0.2
-
 dihydrolipoamide mutant enzyme N286Q Homo sapiens
0.2
-
 dihydrolipoamide mutant N286Q Homo sapiens
0.22
-
 NAD+ wild-type Homo sapiens
0.22
-
 NAD+ wild type enzyme Homo sapiens
0.24
-
 NADH mutant enzyme D320N Homo sapiens
0.24
-
 NADH mutant D320N Homo sapiens
0.3
-
 NAD+ mutant enzyme N286D Homo sapiens
0.3
-
 NAD+ mutant N286D Homo sapiens
0.34
-
 NAD+ mutant enzyme D320N Homo sapiens
0.34
-
 NAD+ mutant D320N Homo sapiens
0.38
-
 dihydrolipoamide wild-type Homo sapiens
0.38
-
 dihydrolipoamide wild type enzyme Homo sapiens
0.46
-
 Lipoamide mutant enzyme D320N Homo sapiens
0.46
-
 Lipoamide mutant D320N Homo sapiens
0.56
-
 Lipoamide wild-type Homo sapiens
0.56
-
 Lipoamide wild type enzyme Homo sapiens
0.7
-
 dihydrolipoamide mutant enzyme N286D Homo sapiens
0.7
-
 dihydrolipoamide mutant N286D Homo sapiens
1.21
-
 dihydrolipoamide mutant enzyme D320N Homo sapiens
1.21
-
 dihydrolipoamide mutant D320N Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 wild-type and mutants, molecular sieving HPLC Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09622
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens
wild-type and mutant proteins, highly purified Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild type enzyme: 586.50 units/mg protein, mutant N286D: 179.65 units/mg protein, mutant N286Q: 143.15 units/mg protein, mutant D320N: 283.10 units/mg protein Homo sapiens
143.1
-
 mutant N286Q Homo sapiens
179.6
-
 mutant N286D Homo sapiens
283.1
-
 mutant D320N Homo sapiens
586.5
-
 wild-type Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH + H+
-
 ?
dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH + H+
-
 r
lipoamide + NADH
-
 Homo sapiens dihydrolipoamide + NAD+
-
 r
lipoamide + NADH + H+
-
 Homo sapiens dihydrolipoamide + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Homo sapiens
dimer wild-type and mutants, molecular sieving HPLC Homo sapiens

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
 Homo sapiens
E3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
67
-
 Lipoamide mutant enzyme D320N Homo sapiens
67
-
 Lipoamide mutant D320N Homo sapiens
107
-
 dihydrolipoamide mutant enzyme D320N Homo sapiens
107
-
 dihydrolipoamide mutant D320N Homo sapiens
116
-
 dihydrolipoamide mutant enzyme N286Q Homo sapiens
116
-
 dihydrolipoamide mutant N286Q Homo sapiens
134
-
 Lipoamide mutant enzyme N286D Homo sapiens
134
-
 Lipoamide mutant N286D Homo sapiens
148
-
 Lipoamide mutant enzyme N286Q Homo sapiens
148
-
 Lipoamide mutant N286Q Homo sapiens
159
-
 dihydrolipoamide mutant enzyme N286D Homo sapiens
159
-
 dihydrolipoamide mutant N286D Homo sapiens
505
-
 dihydrolipoamide wild-type Homo sapiens
505
-
 dihydrolipoamide wild type enzyme Homo sapiens
519
-
 Lipoamide wild-type Homo sapiens
519
-
 Lipoamide wild type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens