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Literature summary for 1.8.1.4 extracted from
- Zinc is a potent inhibitor of thiol oxidoreductase activity and stimulates reactive oxygen species production by lipoamide dehydrogenase (2002), J. Biol. Chem., 277, 10064-10072.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | stimulates the oxygen oxidase activity of the enzyme | Sus scrofa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | product inhibition of the oxygen oxidase activity | Sus scrofa | |
| Zn2+ | reversible binding, competitive to lipoamide, uncompetitive to NADH | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dehydrogenase and oxidase activity kinetics | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| further purification of the commercial preparation by gel filtration | Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping pong mechanism | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Sus scrofa | - |
| heart | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-6,8-thiooctic acid amide + NADH | - |
Sus scrofa | ? + NAD+ | - |
r | |
| lipoamide + NADH | - |
Sus scrofa | dihydrolipoamide + NAD+ | - |
r | |
| O2 + NADH | the enzyme produces reactive oxygen species, i.e. hydrogen peroxide, acting as an oxidase | Sus scrofa | H2O2 + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LADH | - |
Sus scrofa |
| lipoamide dehydrogenase | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Sus scrofa | |
| NAD+ | - |
Sus scrofa | |
| NADH | - |
Sus scrofa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Zn2+ inhibition kinetics and mechanism | Sus scrofa |
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