Cloned (Comment) | Organism |
---|---|
- |
Streptomyces seoulensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces seoulensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
Lipoamide | - |
Streptomyces seoulensis | |
0.4 | - |
1,4-benzoquinone | - |
Streptomyces seoulensis | |
2.15 | - |
lipoic acid | - |
Streptomyces seoulensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Streptomyces seoulensis |
110000 | - |
gel filtration | Streptomyces seoulensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces seoulensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces seoulensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping-pong mechanism | Streptomyces seoulensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.311 | - |
- |
Streptomyces seoulensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 1,4-benzoquinol + NAD+ | - |
? | |
2 ferricyanide + NADH | - |
Streptomyces seoulensis | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2-hydroxy-1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 2-hydroxy-1,4-benzoquinol + NAD+ | - |
? | |
2-methyl-1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 2-methyl-1,4-benzoquinol + NAD+ | - |
? | |
dihydrolipoamide + NAD+ | - |
Streptomyces seoulensis | lipoamide + NADH | - |
? | |
lipoic acid + NADH + H+ | - |
Streptomyces seoulensis | dihydrolipoic acid + NAD+ | - |
r | |
naphthoquinone + NADH | - |
Streptomyces seoulensis | 1,4-naphthoquinol + NAD+ | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADH | - |
Streptomyces seoulensis | ? + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Streptomyces seoulensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
lipoic acid | - |
Streptomyces seoulensis | |
88 | - |
Lipoamide | - |
Streptomyces seoulensis | |
108 | - |
1,4-benzoquinone | - |
Streptomyces seoulensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 FAD per subunit | Streptomyces seoulensis | |
NAD+ | - |
Streptomyces seoulensis | |
NADH | - |
Streptomyces seoulensis |