Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
ferrileghemoglobin | - |
Glycine max | |
0.058 | - |
NADH | - |
Glycine max | |
3.38 | - |
Lipoamide | - |
Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Glycine max | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50027 | - |
x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | O81413 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | enzyme form FlbR-2 | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83.06 | - |
lipoamide reduction | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Glycine max | lipoamide + NADH | - |
? | |
ferrileghemoglobin + NADH + H+ | - |
Glycine max | ferroleghemoglobin + NAD+ | - |
r | |
lipoamide + NADH | - |
Glycine max | dihydrolipoamide + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound to the protein | Glycine max | |
NAD+ | - |
Glycine max | |
NADH | - |
Glycine max |