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Literature summary for 1.8.1.4 extracted from
- Purification of lipoamide dehydrogenase from Ascaris muscle mitochondria and its relationship to NADH:NAD+ transhydrogenase activity (1979), Arch. Biochem. Biophys., 196, 239-247.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | competitive with respect to NAD+ | Ascaris suum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
NADH | NADH:2,6-dichlorophenol indophenol reductase activity | Ascaris suum | |
| 0.009 | - |
NADH | NADH:NAD+ transhydrogenase activity | Ascaris suum | |
| 0.01 | - |
NADH | lipoamide dehydrogenase activity | Ascaris suum | |
| 0.037 | - |
NAD+ | - |
Ascaris suum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Ascaris suum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
2 * 54000, SDS-PAGE | Ascaris suum |
| 105000 | - |
gel filtration | Ascaris suum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90.2 | - |
- |
Ascaris suum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 6 months | Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Ascaris suum | lipoamide + NADH | - |
? | |
| dihydrolipoamide + NAD+ | - |
Ascaris suum | lipoamide + NADH | - |
r | |
| additional information | NADH:NAD+ transhydrogenase activity | Ascaris suum | ? | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Ascaris suum | ? + NAD+ | - |
? | |
| thio-NAD+ + NADH | - |
Ascaris suum | thio-NADH + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54000, SDS-PAGE | Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
reduction of lipoamide | Ascaris suum |
| 7.4 | - |
reduction of 2,6-dichlorophenol indophenol | Ascaris suum |
| 7.5 | - |
reduction of thio-NAD+ | Ascaris suum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 FAD per subunit | Ascaris suum | |
| NAD+ | - |
Ascaris suum | |
| NADH | - |
Ascaris suum | |
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