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Literature summary for 1.8.1.2 extracted from
- Purification and characterization of Escherichia coli sulfite reductase and its application in surimi processing (2002), J. Food Sci., 67, 3329-3334.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | quality determination of surimi, purified enzyme increases the reactive SH and gel strength of surimi prepared from frozen mackerel, processing of surimi-based products | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Escherichia coli |  |
| Ca2+ | strong inhibition | Escherichia coli | |
| Cd2+ | - |
Escherichia coli | |
| Co2+ | strong inhibition | Escherichia coli | |
| Cu2+ | strong inhibition | Escherichia coli | |
| Fe2+ | strong inhibition | Escherichia coli | |
| Fe3+ | strong inhibition | Escherichia coli | |
| Hg2+ | strong inhibition | Escherichia coli | |
| iodoacetic acid | - |
Escherichia coli | |
| KCN | complete inhibition at 1 mM | Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| additional information | inhibitory potency of metal ions, overview, no inhibition by Na+, K+, and Mg2+ | Escherichia coli | |
| NEM | - |
Escherichia coli | |
| PCMB | complete inhibition at 1 mM | Escherichia coli | |
| PMSF | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 579.5fold to homogeneity by ammoniums sulfate fractionation, DEAE ion exchange chromatography, and gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.02 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + NADPH | - |
Escherichia coli | hydrogen sulfide + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-sulfite reductase | - |
Escherichia coli |
| sulfite reductase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 75% activity at pH 6.5 and pH 8.0 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 75% activity at pH 6.5 and pH 8.0 after 30 min at 25°C | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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