Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30686 | - |
1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence | Pyrococcus furiosus |
52598 | - |
1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U195 and Q8U194 | Q8U195: subunit alpha, and Q8U194: subunit beta | - |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 52598 (alpha-subunit, SudA) + 1 * 30686 (beta-subunit, SudB), calculated from sequence | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | both the alpha-subunit and the beta-subunit contains one FAD | Pyrococcus furiosus | |
Fe-S center | the alpha-subunit carries a [2Fe-2S]2+,+ prosthetic group. It exhibits EPR g-values, 2.035, 1.908, 1.786, and reduction potential, Em,8 = +80 mV, reminiscent of Rieske-type clusters. Comparative sequence analysis indicates that this cluster is coordinated by a novel motif of one Asp and three Cys ligands. The beta-subunit carries a [3Fe-4S]+,0 cluster, and a [4Fe-4S]2+,+ cluster. The 3Fe cluster has an unusually high reduction potential, Em,8 = +230 mV. The reduced 4Fe cluster exhibits a complex EPR signal, presumably resulting from magnetic interaction of its S = 1/2 spin with the S=2 spin of the reduced 3Fe cluster. The 4Fe cluster can be reduced with deazaflavin/EDTA/light but not with sodium dithionite, however, it is readily reduced with NADPH | Pyrococcus furiosus | |
NADPH | both the alpha-subunit and the beta-subunit carry one putative nucleotide binding site for NADPH | Pyrococcus furiosus |