Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.19 extracted from

  • Ma, K.; Adams, M.W.
    Sulfide dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus: a new multifunctional enzyme involved in the reduction of elemental sulfur (1994), J. Bacteriol., 176, 6509-6517.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
NADPH pH 8.0, 80°C Pyrococcus furiosus
1.25
-
(sulfide)n+1 pH 8.0, 80°C Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pyrococcus furiosus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains no metal Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 52000 + 1 * 29000, Pyrococcus furiosus
52000
-
1 * 52000 + 1 * 29000, Pyrococcus furiosus
90000
-
gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(sulfide)n+1 + NADPH + H+ Pyrococcus furiosus NADH is not utilized as an electron donor for polysulfide reduction. Polysulfide reduction is catalyzed with reduced ferredoxin as an electron donor. However, the rate of sulfide production, at least in vitro, is much lower than when the enzyme uses NADPH to provide reductant for polysulfide reduction hydrogen sulfide + (sulfide)n + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U195 and Q8U194 Q8U195: subunit alpha, and Q8U194: subunit beta
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
pH 8.0, 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(sulfide)n+1 + NADPH + H+ NADH is not utilized as an electron donor for polysulfide reduction. Polysulfide reduction is catalyzed with reduced ferredoxin as an electron donor. However, the rate of sulfide production, at least in vitro, is much lower than when the enzyme uses NADPH to provide reductant for polysulfide reduction Pyrococcus furiosus hydrogen sulfide + (sulfide)n + NADP+
-
?
colloidal sulfur + NADPH + H+ the relative activity is about 40% compared to the activity with polysulfide Pyrococcus furiosus ?
-
?
additional information the enzyme also catalyzes ferredoxin:NADP+ reductase activity Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 52000 + 1 * 29000, Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
SuDH
-
Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
82
-
the enzyme shows a 50% increase in activity after 12 h Pyrococcus furiosus
95
-
half-life: 12 h Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Fe-S center the enzyme contains approximately four iron-sulfur centers, 11 iron and 6 acid-labile sulfide atoms per mol Pyrococcus furiosus
flavin enzyme contains flavin Pyrococcus furiosus
NADPH NADH was not utilized as an electron donor for polysulfide reduction Pyrococcus furiosus