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Literature summary for 1.8.1.16 extracted from

  • Vergauwen, B.; Pauwels, F.; Jacquemotte, F.; Meyer, T.E.; Cusanovich, M.A.; Bartsch, R.G.; van Beeumen, J.J.
    Characterization of glutathione amide reductase from Chromatium gracile. Identification of a novel thiol peroxidase (Prx/Grx) fueled by glutathione amide redox cycling (2001), J. Biol. Chem., 276, 20890-20897.
    View publication on PubMed
Search for more literature for 1.8.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Marichromatium gracile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0132
-
 NADH pH 7.1, 25°C Marichromatium gracile
0.097
-
 glutathione amide disulfide pH 7.1, 25°C Marichromatium gracile
1.98
-
 NADPH pH 7.1, 25°C Marichromatium gracile
6.9
-
 glutathione disulfide pH 7.1, 25°C Marichromatium gracile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49028
-
 2 * 49028, calculation from sequence Marichromatium gracile
49030
-
 2 * 49030, nanospray ionization mass spectrometry Marichromatium gracile
98000
-
 gel filtration Marichromatium gracile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione amide disulfide + NADH + H+ Marichromatium gracile
-
 2 glutathione amide + NAD+
-
 ?
glutathione amide disulfide + NADH + H+ Marichromatium gracile DSM 1712
-
 2 glutathione amide + NAD+
-
 ?
glutathione amide disulfide + NADPH + H+ Marichromatium gracile
-
 2 glutathione amide + NADP+
-
 ?
glutathione amide disulfide + NADPH + H+ Marichromatium gracile DSM 1712
-
 2 glutathione amide + NADP+
-
 ?
glutathione disulfide + NADH + H+ Marichromatium gracile
-
 2 glutathione + NAD+
-
 ?
glutathione disulfide + NADH + H+ Marichromatium gracile DSM 1712
-
 2 glutathione + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Marichromatium gracile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Marichromatium gracile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione amide disulfide + NADH + H+
-
 Marichromatium gracile 2 glutathione amide + NAD+
-
 ?
glutathione amide disulfide + NADH + H+
-
 Marichromatium gracile DSM 1712 2 glutathione amide + NAD+
-
 ?
glutathione amide disulfide + NADPH + H+
-
 Marichromatium gracile 2 glutathione amide + NADP+
-
 ?
glutathione amide disulfide + NADPH + H+
-
 Marichromatium gracile DSM 1712 2 glutathione amide + NADP+
-
 ?
glutathione disulfide + NADH + H+
-
 Marichromatium gracile 2 glutathione + NAD+
-
 ?
glutathione disulfide + NADH + H+
-
 Marichromatium gracile DSM 1712 2 glutathione + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 49028, calculation from sequence Marichromatium gracile
dimer 2 * 49030, nanospray ionization mass spectrometry Marichromatium gracile

Synonyms

Synonyms Comment Organism
GAR
-
 Marichromatium gracile
garB
-
 Marichromatium gracile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 NADPH pH 7.1, 25°C Marichromatium gracile
146
-
 glutathione disulfide pH 7.1, 25°C Marichromatium gracile
186.8
-
 NADH pH 7.1, 25°C Marichromatium gracile
249.7
-
 glutathione amide disulfide pH 7.1, 25°C Marichromatium gracile

Cofactor

Cofactor Comment Organism Structure
FAD formation of a charge-transfer complex between one of the newly reduced active site thiols and the oxidized FAD Marichromatium gracile
NADH preferred cofactor Marichromatium gracile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
 NADPH pH 7.1, 25°C Marichromatium gracile
21.2
-
 glutathione disulfide pH 7.1, 25°C Marichromatium gracile
2574
-
 glutathione amide disulfide pH 7.1, 25°C Marichromatium gracile
14150
-
 NADH pH 7.1, 25°C Marichromatium gracile