Cloned (Comment) | Organism |
---|---|
- |
Leishmania infantum |
Crystallization (Comment) | Organism |
---|---|
- |
Leishmania infantum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine | most active inhibitor | Leishmania infantum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
trypanothione disulfide | in the absence of 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
0.053 | - |
trypanothione disulfide | in the presence of 0.01 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
0.075 | - |
trypanothione disulfide | in the presence of 0.03 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH + H+ | Leishmania infantum | - |
trypanothione + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | A4HSF7 | - |
- |
Purification (Comment) | Organism |
---|---|
in complex with inhibitor 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine | Leishmania infantum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania infantum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH + H+ | - |
Leishmania infantum | trypanothione + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | - |
Leishmania infantum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.4 | - |
trypanothione disulfide | in the absence of 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
18 | - |
trypanothione disulfide | in the presence of 0.01 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
19 | - |
trypanothione disulfide | in the presence of 0.03 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leishmania infantum | |
NADPH | - |
Leishmania infantum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine | at 20°C, pH not specified in the publication | Leishmania infantum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
trypanothione disulfide | in the presence of 0.03 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
340 | - |
trypanothione disulfide | in the presence of 0.01 mM 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum | |
470 | - |
trypanothione disulfide | in the absence of 4-((1-(4-ethylphenyl)-2-methyl-5-(4-(methylthio)phenyl)-1H-pyrrol-3-yl)methyl)thiomorpholine, at 20°C, pH not specified in the publication | Leishmania infantum |