Literature summary for 1.8.1.12 extracted from

Shukla, A.K.; Patra, S.; Dubey, V.K.
Biophysical and folding parameters of trypanothione reductase from Leishmania infantum (2011), Appl. Biochem. Biotechnol., 165, 13-23.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Leishmania infantum

General Stability

General Stability	Organism
the enzyme retains 76.02% and 41.17% activity in the presence of 2 M urea after 1 and 24 h, respectively. The enzyme regains activity up to 54.91% after denaturation in 8 M urea. The enzyme is active even after 24 h of guanidine hydrochloride treatment and contains 74.67% and 13.33% activity in 2 M after 1 and 24 h, respectively. Once denatured with 6 M guanidine hydrochloride, the protein regains 45.73% once guanidine hydrochloride is dialyzed	Leishmania infantum

Organism

the enzyme retains 76.02% and 41.17% activity in the presence of 2 M urea after 1 and 24 h, respectively. The enzyme regains activity up to 54.91% after denaturation in 8 M urea. The enzyme is active even after 24 h of guanidine hydrochloride treatment and contains 74.67% and 13.33% activity in 2 M after 1 and 24 h, respectively. Once denatured with 6 M guanidine hydrochloride, the protein regains 45.73% once guanidine hydrochloride is dialyzed

Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trypanothione disulfide + NADPH + H+	Leishmania infantum	-	trypanothione + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania infantum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity column chromatography	Leishmania infantum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trypanothione disulfide + NADPH + H+	-	Leishmania infantum	trypanothione + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
trypanothione reductase	-	Leishmania infantum
TryR	-	Leishmania infantum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Leishmania infantum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	11	less than 15% activity at pH 6.0, about 50% activity at pH 6.5, about 80% activity at pH 7.0, 100% activity at pH 7.5, about 35% activity at pH 8.0, around 30% activity between pH 8.5 and 11.0, no activity at pH 12.0 and less than pH 5.0	Leishmania infantum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Leishmania infantum
NADPH	-	Leishmania infantum