Application | Comment | Organism |
---|---|---|
medicine | enzyme is a target for development of antileishmanial drugs | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as soluble glutathione-S-transferase fusion protein | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Melarsen oxide | over 95% inhibition at 0.05 mM | Leishmania donovani | |
additional information | no inhibition by melarsoprol | Leishmania donovani | |
nifurtimox | - |
Leishmania donovani | |
nitrofurazone | - |
Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism | Leishmania donovani | |
0.02 | - |
NADPH | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani | |
0.05 | - |
trypanothione disulfide | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54668 | - |
2 * 54668, recombinant enzyme, mass spectrometry and SDS-PAGE | Leishmania donovani |
110000 | - |
recombinant enzyme, native PAGE | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania donovani | phylogenetic relationship | ? | - |
? | |
additional information | Leishmania donovani Dd8 | phylogenetic relationship | ? | - |
? | |
trypanothione disulfide + NADPH | Leishmania donovani | - |
trypanothione + NADP+ | - |
? | |
trypanothione disulfide + NADPH | Leishmania donovani Dd8 | - |
trypanothione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q95PT6 | - |
- |
Leishmania donovani Dd8 | Q95PT6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble glutathione-S-transferase fusion protein from Escherichia coli strain BL21(DE3) to homogeneity, 20fold | Leishmania donovani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.53 | - |
purified recombinant enzyme | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phylogenetic relationship | Leishmania donovani | ? | - |
? | |
additional information | no activity with oxidized glutathione of the recombinant enzyme | Leishmania donovani | ? | - |
? | |
additional information | phylogenetic relationship | Leishmania donovani Dd8 | ? | - |
? | |
additional information | no activity with oxidized glutathione of the recombinant enzyme | Leishmania donovani Dd8 | ? | - |
? | |
trypanothione disulfide + NADPH | - |
Leishmania donovani | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + NADPH | - |
Leishmania donovani Dd8 | trypanothione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54668, recombinant enzyme, mass spectrometry and SDS-PAGE | Leishmania donovani |
More | amino acid sequence of the active site | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Leishmania donovani |
trypanothione reductase | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.18 | - |
trypanothione disulfide | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | amino acid sequence of the binding site | Leishmania donovani | |
NADPH | amino acid sequence of the binding site | Leishmania donovani |