Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Application | Comment | Organism |
---|---|---|
medicine | potential target for drug design against trypanosomiasis | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) | recombinant enzyme | Trypanosoma cruzi | |
clomipramine | recombinant enzyme | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) | recombinant enzyme | Trypanosoma cruzi | |
0.045 | - |
trypanothione disulfide | recombinant enzyme | Trypanosoma cruzi | |
0.3 | - |
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) | recombinant enzyme | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
137 | - |
recombinant purified enzyme | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) + NADPH + H+ | - |
Trypanosoma cruzi | ? | - |
r | |
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+ | - |
Trypanosoma cruzi | ? | - |
? | |
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+ | - |
Trypanosoma cruzi | ? | - |
r | |
additional information | reduces 5,5'-dithiobis(2-nitrobenzamides) | Trypanosoma cruzi | ? | - |
? | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
125 | - |
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) | recombinant enzyme | Trypanosoma cruzi | |
125 | - |
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) | recombinant enzyme | Trypanosoma cruzi | |
240 | - |
trypanothione disulfide | recombinant enzyme | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
assay at | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
clomipramine | recombinant enzyme, with 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) | Trypanosoma cruzi |