Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma congolense |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Trypanosoma congolense |
Protein Variants | Comment | Organism |
---|---|---|
E201D | by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity | Trypanosoma congolense |
E201Q | by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity | Trypanosoma congolense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine nucleotide | - |
Trypanosoma congolense | |
NADP+ | - |
Trypanosoma congolense | |
oxidized 3-acetylpyridine adenine nucleotide | wild-type and mutant E201D and E201Q | Trypanosoma congolense | |
thio-NADP+ | wild-type and mutant E201D and E201Q | Trypanosoma congolense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
NADPH | - |
Trypanosoma congolense | |
0.0015 | 0.002 | NADPH | mutant E201D and E201Q | Trypanosoma congolense | |
0.0023 | 0.0066 | beta-thio-NADPH | mutant E201D and E201Q | Trypanosoma congolense | |
0.003 | - |
3-acetylpyridine adenine dinucleotide | mutant E201D and E201Q | Trypanosoma congolense | |
0.0039 | - |
3-acetylpyridine adenine dinucleotide | - |
Trypanosoma congolense | |
0.0113 | - |
beta-thio-NADPH | - |
Trypanosoma congolense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH | Trypanosoma congolense | physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide | trypanothione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma congolense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants recombinant from Escherichia coli | Trypanosoma congolense |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ | hydride ion transfer reaction of the pro-S-hydrogen of NADPH to N5 of FAD, redox potentials | Trypanosoma congolense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADPH + H+ | - |
Trypanosoma congolense | ? | - |
? | |
9,10-phenanthrenequinone + NADPH + H+ | - |
Trypanosoma congolense | ? | - |
? | |
trypanothione disulfide + beta-thio-NADPH | wild-type and mutant enzymes | Trypanosoma congolense | trypanothione + beta-thio-NADP+ | - |
? | |
trypanothione disulfide + NADPH | - |
Trypanosoma congolense | trypanothione + NADP+ | - |
ir | |
trypanothione disulfide + NADPH | physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide | Trypanosoma congolense | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + reduced 3-acetylpyridine adenine dinucleotide | wild-type and mutant enzymes | Trypanosoma congolense | trypanothione + oxidized 3-acetylpyridine adenine dinucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Trypanosoma congolense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
9,10-phenanthrenequinone | - |
Trypanosoma congolense | |
4.8 | - |
1,4-benzoquinone | - |
Trypanosoma congolense | |
5.1 | - |
trypanothione disulfide | mutant E201Q | Trypanosoma congolense | |
5.3 | - |
trypanothione disulfide | mutant E201D | Trypanosoma congolense | |
9.4 | - |
9,10-phenanthrenequinone | mutant E201Q | Trypanosoma congolense | |
13.6 | - |
1,4-benzoquinone | mutant E201D | Trypanosoma congolense | |
24.5 | - |
1,4-benzoquinone | mutant E201Q | Trypanosoma congolense | |
29 | - |
9,10-phenanthrenequinone | mutant E201D | Trypanosoma congolense | |
122 | - |
trypanothione disulfide | - |
Trypanosoma congolense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | - |
Trypanosoma congolense | |
beta-thio-NADPH | - |
Trypanosoma congolense | |
FAD | - |
Trypanosoma congolense | |
NADPH | - |
Trypanosoma congolense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
3-acetylpyridine adenine nucleotide | mutant E201Q | Trypanosoma congolense | |
0.0033 | - |
3-acetylpyridine adenine nucleotide | mutant E201D | Trypanosoma congolense | |
0.0054 | - |
thio-NADP+ | mutant E201D, slopes | Trypanosoma congolense | |
0.0084 | - |
3-acetylpyridine adenine nucleotide | - |
Trypanosoma congolense | |
0.011 | - |
NADP+ | wild-type and mutant E201D | Trypanosoma congolense | |
0.011 | - |
thio-NADP+ | mutant E201Q | Trypanosoma congolense | |
0.014 | - |
NADP+ | mutant E201Q | Trypanosoma congolense | |
0.051 | - |
thio-NADP+ | mutant E201D, intercepts | Trypanosoma congolense |