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Literature summary for 1.8.1.12 extracted from

  • Zheng, R.; Cenas, N.; Blanchard, J.S.
    Catalytic and potentiometric characterization of E201D and E201Q mutants of Trypanosoma congolense trypanothione reductase (1995), Biochemistry, 34, 12697-12703.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma congolense

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Trypanosoma congolense

Protein Variants

Protein Variants Comment Organism
E201D by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity Trypanosoma congolense
E201Q by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity Trypanosoma congolense

Inhibitors

Inhibitors Comment Organism Structure
3-acetylpyridine adenine nucleotide
-
Trypanosoma congolense
NADP+
-
Trypanosoma congolense
oxidized 3-acetylpyridine adenine nucleotide wild-type and mutant E201D and E201Q Trypanosoma congolense
thio-NADP+ wild-type and mutant E201D and E201Q Trypanosoma congolense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
NADPH
-
Trypanosoma congolense
0.0015 0.002 NADPH mutant E201D and E201Q Trypanosoma congolense
0.0023 0.0066 beta-thio-NADPH mutant E201D and E201Q Trypanosoma congolense
0.003
-
3-acetylpyridine adenine dinucleotide mutant E201D and E201Q Trypanosoma congolense
0.0039
-
3-acetylpyridine adenine dinucleotide
-
Trypanosoma congolense
0.0113
-
beta-thio-NADPH
-
Trypanosoma congolense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypanothione disulfide + NADPH Trypanosoma congolense physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide trypanothione + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma congolense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutants recombinant from Escherichia coli Trypanosoma congolense

Reaction

Reaction Comment Organism Reaction ID
trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ hydride ion transfer reaction of the pro-S-hydrogen of NADPH to N5 of FAD, redox potentials Trypanosoma congolense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NADPH + H+
-
Trypanosoma congolense ?
-
?
9,10-phenanthrenequinone + NADPH + H+
-
Trypanosoma congolense ?
-
?
trypanothione disulfide + beta-thio-NADPH wild-type and mutant enzymes Trypanosoma congolense trypanothione + beta-thio-NADP+
-
?
trypanothione disulfide + NADPH
-
Trypanosoma congolense trypanothione + NADP+
-
ir
trypanothione disulfide + NADPH physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide Trypanosoma congolense trypanothione + NADP+
-
?
trypanothione disulfide + reduced 3-acetylpyridine adenine dinucleotide wild-type and mutant enzymes Trypanosoma congolense trypanothione + oxidized 3-acetylpyridine adenine dinucleotide
-
?

Synonyms

Synonyms Comment Organism
TR
-
Trypanosoma congolense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
9,10-phenanthrenequinone
-
Trypanosoma congolense
4.8
-
1,4-benzoquinone
-
Trypanosoma congolense
5.1
-
trypanothione disulfide mutant E201Q Trypanosoma congolense
5.3
-
trypanothione disulfide mutant E201D Trypanosoma congolense
9.4
-
9,10-phenanthrenequinone mutant E201Q Trypanosoma congolense
13.6
-
1,4-benzoquinone mutant E201D Trypanosoma congolense
24.5
-
1,4-benzoquinone mutant E201Q Trypanosoma congolense
29
-
9,10-phenanthrenequinone mutant E201D Trypanosoma congolense
122
-
trypanothione disulfide
-
Trypanosoma congolense

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine adenine dinucleotide
-
Trypanosoma congolense
beta-thio-NADPH
-
Trypanosoma congolense
FAD
-
Trypanosoma congolense
NADPH
-
Trypanosoma congolense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0017
-
3-acetylpyridine adenine nucleotide mutant E201Q Trypanosoma congolense
0.0033
-
3-acetylpyridine adenine nucleotide mutant E201D Trypanosoma congolense
0.0054
-
thio-NADP+ mutant E201D, slopes Trypanosoma congolense
0.0084
-
3-acetylpyridine adenine nucleotide
-
Trypanosoma congolense
0.011
-
NADP+ wild-type and mutant E201D Trypanosoma congolense
0.011
-
thio-NADP+ mutant E201Q Trypanosoma congolense
0.014
-
NADP+ mutant E201Q Trypanosoma congolense
0.051
-
thio-NADP+ mutant E201D, intercepts Trypanosoma congolense