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Literature summary for 1.8.1.12 extracted from
- Trypanothione reductase from Trypanosoma cruzi. Catalytic properties of the enzyme and inhibition studies with trypanocidal compounds (1989), Eur. J. Biochem., 180, 267-272.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-Bis(2-chloroethyl)-1-nitrosourea | - |
Trypanosoma cruzi |  |
| 2,3-Bis(3-(2-amidinohydrazono)-butyl)-1,4-naphthoquinone dihydrochloride | - |
Trypanosoma cruzi | |
| 2-(5-nitro-2-furanylmethylidene)-N,N'-(1,4-piperazinediylbis(1,3-propanediyl))bishydrazinecarboximidamide tetrahydrobromide | - |
Trypanosoma cruzi | |
| additional information | increasing ionic strength leads to inhibition, e.g. with NaCl, KCl, (NH4)SO4, HEPES | Trypanosoma cruzi | |
| nifurtimox | - |
Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | 0.007 | NADPH | - |
Trypanosoma cruzi | |
| 0.018 | 0.14 | trypanothione disulfide | dependent on buffer system | Trypanosoma cruzi | |
| 0.022 | 0.275 | glutathionylspermidine disulfide | dependent on buffer system | Trypanosoma cruzi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
r | |
| trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
glutathionylspermidine disulfide + NADPH, additional 2 minor optima at pH 7.0 and pH 8.0 | Trypanosoma cruzi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Trypanosoma cruzi | |
| NADPH | - |
Trypanosoma cruzi | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
nifurtimox | - |
Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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