Cloned (Comment) | Organism |
---|---|
mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli | Homo sapiens |
overexpression in Escherichia coli | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
recombinant glutathione-trypanothione reductase-like enzyme, hanging-drop vapour diffusion method, 0.1 M potasssium phosphate, pH 8.0 | Homo sapiens |
substrate-binding and structure analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A34E/R37W | glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione disulfide than with glutathione | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
trypanothione disulfide | - |
Trypanosoma cruzi | |
0.5 | - |
trypanothione disulfide | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + NADPH | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens | trypanothione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Homo sapiens |
TR | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.8 | - |
trypanothione disulfide | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens |
7.25 | 7.75 | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
FAD | - |
Trypanosoma cruzi | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Trypanosoma cruzi |