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Literature summary for 1.8.1.12 extracted from

  • Stoll, V.S.; Simpson, S.J.; Krauth-Siegel, R.L.; Walsh, C.T.; Pai, E.F.
    Glutathione reductase turned into trypanothione reductase: structural analysis of an engineered change in substrate specificity (1997), Biochemistry, 36, 6437-6447.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli Homo sapiens
overexpression in Escherichia coli Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant glutathione-trypanothione reductase-like enzyme, hanging-drop vapour diffusion method, 0.1 M potasssium phosphate, pH 8.0 Homo sapiens
substrate-binding and structure analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
A34E/R37W glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione disulfide than with glutathione Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
trypanothione disulfide
-
Trypanosoma cruzi
0.5
-
trypanothione disulfide recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypanothione disulfide + NADPH
-
Trypanosoma cruzi trypanothione + NADP+
-
?
trypanothione disulfide + NADPH recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens trypanothione + NADP+
-
?

Synonyms

Synonyms Comment Organism
TR
-
Homo sapiens
TR
-
Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.8
-
trypanothione disulfide recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
recombinant glutathione-trypanothione reductase-like enzyme Homo sapiens
7.25 7.75
-
Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
FAD
-
Trypanosoma cruzi
NADPH
-
Homo sapiens
NADPH
-
Trypanosoma cruzi