Any feedback?
Literature summary for 1.7.7.2 extracted from
- Purification and properties of a dissimilatory nitrate reductase from Haloferax denitrificans (1991), Arch. Biochem. Biophys., 288, 380-385.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is most stable in the absence of salt | Haloferax denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | competitive inhibitor with respect to nitrate | Haloferax denitrificans |  |
| Chlorate | competitive inhibitor with respect to nitrate | Haloferax denitrificans | |
| cyanide | preincubating the enzyme with cyanide increases the extent of inhibition which in turn is intensified when dithionite is present. Although cyanide is a noncompetitive inhibitor with respect to nitrate, nitrate protects against inhibition | Haloferax denitrificans | |
| thiocyanate | 10 mM, 53% inhibition. Preincubating for 1 h does not increase the inhibition | Haloferax denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
reduced methyl viologen | 37°C, pH 7.2 | Haloferax denitrificans | |
| 0.19 | - |
nitrate | 37°C, pH 7.2 | Haloferax denitrificans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Haloferax denitrificans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | maximum activity occurrs in the absence of NaCl and decreases with increasing concentrations of NaCl so that the activity is 46% of maximum when the enzyme is assayed in the presence of 4 M NaCl. Replacing NaCl with KCl does not significantly change the response | Haloferax denitrificans | |
| NaCl | maximum activity occurrs in the absence of NaCl and decreases with increasing concentrations of NaCl so that the activity is 46% of maximum when the enzyme is assayed in the presence of 4 M NaCl. Replacing NaCl with KCl does not significantly change the response | Haloferax denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
| 116000 | - |
2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
| 380000 | - |
gel filtration | Haloferax denitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax denitrificans | - |
- |
- |
| Haloferax denitrificans DSM 4425 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
37°C, pH 7.2 | Haloferax denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -19°C, 3 months, enzyme loses no activity | Haloferax denitrificans |
| 4°C, 8 days, in the absence of NaCl, little if any loss of activity | Haloferax denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorate + reduced acceptor | - |
Haloferax denitrificans | chlorite + acceptor | - |
? | |
| chlorate + reduced acceptor | - |
Haloferax denitrificans DSM 4425 | chlorite + acceptor | - |
? | |
| nitrate + reduced methyl viologen | - |
Haloferax denitrificans | nitrite + methyl viologen | - |
? | |
| nitrate + reduced methyl viologen | - |
Haloferax denitrificans DSM 4425 | nitrite + methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dissimilatory nitrate reductase | - |
Haloferax denitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Haloferax denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Haloferax denitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the activities at pH 6.0 and pH 8.0 are 83% and 81% of the activity at pH 7.2 | Haloferax denitrificans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
azide | 37°C, pH 7.2 | Haloferax denitrificans | |
| 0.5 | - |
cyanide | 37°C, pH 7.2 | Haloferax denitrificans | |
| 0.8 | - |
Chlorate | 37°C, pH 7.2 | Haloferax denitrificans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
