Cloned (Comment) | Organism |
---|---|
expression of native and His-tagged nitrate reductase in Escherichia coli | Synechococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength | Synechococcus sp. | |
MgSO4 | inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength | Synechococcus sp. | |
N-acetylsuccinimide | 115fold excess, loss of more than 80% and 5-10% loss of ferredoxin-dependent and methylviologen dependent activity after 1 h, respectively | Synechococcus sp. | |
NaCl | more than 80% inhibition above 200 mM, inhibition of nitrate reductase-ferredoxin complex formation at high ionic strength | Synechococcus sp. | |
Phenylglyoxal | 76fold excess, approx. 60% and 20% loss of ferredoxin dependent and methylviologen dependent activity after 2 h, respectively | Synechococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
oxidized ferredoxin | spinach ferredoxin | Synechococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains one iron sulfur cluster | Synechococcus sp. | |
Molybdenum | enzyme probably contains a bis-molybdopterin guanine dinucleotide | Synechococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, deduced from nucleotide sequence | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced ferredoxin | Synechococcus sp. | - |
nitrite + H2O + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
PCC 7942 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged nitrate reductase, Ni2+-affinity column | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced ferredoxin | - |
Synechococcus sp. | nitrite + H2O + oxidized ferredoxin | - |
? | |
nitrate + reduced ferredoxin | spinach ferredoxin or Anabaena ferredoxin | Synechococcus sp. | nitrite + H2O + oxidized ferredoxin | - |
? | |
nitrate + reduced methylviologen | - |
Synechococcus sp. | nitrite + H2O + oxidized methylviologen | - |
? | |
nitrite + H2O + oxidized ferredoxin | - |
Synechococcus sp. | nitrate + reduced ferredoxin + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, deduced from nucleotide sequence | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdopterin | - |
Synechococcus sp. |