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Literature summary for 1.7.7.1 extracted from

  • Vega, J.M.; Kamin, H.
    Spinach nitrite reductase. Purification and properties of a siroheme-containing iron-sulfur enzyme (1977), J. Biol. Chem., 252, 896-909.
    View publication on PubMed
Search for more literature for 1.7.7.1

Inhibitors

Inhibitors Comment Organism Structure
CO forms a complex with the reduced enzyme Spinacia oleracea
cyanide competitive inhibition with respect to nitrite Spinacia oleracea
p-chloromercuribenzoate nitrite partially protects the inhibition Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains about 2 mol of siroheme per mol of enzyme, 3 mol of iron per mol of enzyme (MW 61000), one in siroheme, one (Fe2-S2) Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Spinacia oleracea
60000 63000
-
 Spinacia oleracea
62690
-
 amino acid composition Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
107.8
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced ferredoxin
-
 Spinacia oleracea ammonia + oxidized ferredoxin
-
 ?

Cofactor

Cofactor Comment Organism Structure
siroheme prosthetic group Spinacia oleracea
siroheme 1 mol siroheme per mol of enzyme Spinacia oleracea