Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | forms a complex with the reduced enzyme | Spinacia oleracea | |
cyanide | competitive inhibition with respect to nitrite | Spinacia oleracea | |
p-chloromercuribenzoate | nitrite partially protects the inhibition | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains about 2 mol of siroheme per mol of enzyme, 3 mol of iron per mol of enzyme (MW 61000), one in siroheme, one (Fe2-S2) | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Spinacia oleracea |
60000 | 63000 | - |
Spinacia oleracea |
62690 | - |
amino acid composition | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
107.8 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | prosthetic group | Spinacia oleracea | |
siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea |