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Literature summary for 1.7.3.6 extracted from

  • Moir, J.W.B.; Wehrfritz, J.M.; Spiro, S.; Richardson, D.J.
    The biochemical characterization of a novel non-heme-iron hydroxylamine oxidase from Paracoccus denitrificans GB17 (1996), Biochem. J., 319, 823-827.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
cyanide the cyanide-sensitive form of the enzyme exists only during turnover Paracoccus denitrificans
EDTA 1 mM, in 10 mM Tris/HCl, pH 8.0, almost complete inhibition Paracoccus denitrificans
NaCl 15 mM, 50% inhibition of activity with horse heart cytochrome, activity with pseudoazurin or cytochrome c550 is inhibited only above 100 mM Paracoccus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 3-5 nonhaem, non-iron-sulfur iron atoms as prosthetic groups, predominantly coordinated by carboxylate ligands Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
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strain GB17, heterotrophic nitrifier
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + ferricytochrome c550 under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction Paracoccus denitrificans nitroxyl + ferrocytochrome c550 + H+
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?
hydroxylamine + O2 horse heart cytochrome. Under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction Paracoccus denitrificans nitrite + H2O + H+
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?
hydroxylamine + pseudoazurin under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction Paracoccus denitrificans nitrite + reduced pseudoazurin
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?

Synonyms

Synonyms Comment Organism
hydroxylamine oxidase
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Paracoccus denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
hydroxylamine pH 8, temperature not specified in the publication Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
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activity with horse heart cytochrome c Paracoccus denitrificans
10
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activity with pseudoazurin Paracoccus denitrificans
11
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activity with cytochrome c550 continues to increase to pH 11 Paracoccus denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
7.3 11.5 pH 7.3: about 35% of maximal activity, pH 11.5: about 50% of maximal activity activity with pseudoazurin Paracoccus denitrificans
7.6 10 pH 7.6: about 50% of maximal activity, pH 10.0: about 35% of maximal activity with horse heart cytochrome c Paracoccus denitrificans
8 11 activity with cytochrome c550 continues to increase to pH 11, activity at pH 8 is about 50% of the activity at pH 11 Paracoccus denitrificans